A Mouse Model of Acute Hemolytic Transfusion Reactions

doi:10.3969/j.issn.1671-2587.2023.01.011

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2023, Vol. 25 ›› Issue (1): 63-68.DOI: 10.3969/j.issn.1671-2587.2023.01.011

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A Mouse Model of Acute Hemolytic Transfusion Reactions

LIU Zhi-xin, WU Xiao-shuang, MENG Wei-cheng, et al

Department of Transfusion Medicine, the First Affiliated Hospital of Air Force Military Medical University, Shaanxi Province, Xi'an 710032

Received:2022-09-20 Published:2023-02-20

Abstract

Abstract: Objective To establish a mouse model of acute hemolytic transfusion reactions (AHTRs) to explore the mechanism and intervention of preventing AHTRs and ensure safety of blood transfusion. Methods In vitro blood compatibility of mice and humans were verified by cross matching testing. Then, the mice model of AHTRs was established by intravenously injecting human O-type plasma into mice. The effectiveness of AHTRs model was verified by routine detection of red blood cell count and morphology in peripheral blood, serological detection of red blood cell sensitization, pathological histological section staining and biochemical analysis of kidney and liver injury levels. Results The mice who received mismatched plasma transfusions developed hematuria. Red blood cell count reduced and morphogenesis deformity occurred. Irregular red blood cell antibodies were positive. The levels of plasma free hemoglobin increased. Free hemoglobin exacerbated liver and kidney injury through blood circulation. Conclusion A mouse model of acute hemolytic transfusion reaction was established successfully.

Key words: Blood transfusion, Acute hemolysis, Animal models

CLC Number:

R332

LIU Zhi-xin, WU Xiao-shuang, MENG Wei-cheng, et al. A Mouse Model of Acute Hemolytic Transfusion Reactions[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(1): 63-68.

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A Mouse Model of Acute Hemolytic Transfusion Reactions

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