Abstract: Objective To investigate the hemolysis rate of red cell suspensions during the late storage period from donors with different genders and ages, and thus provide data beneficial to scientific research on blood service and blood quality control. Methods From June 2012 to June 2021, 400 bags of 1 unit suspended red blood cells were randomly sampled, and the hemolysis rate was measured by aseptic sampling on day 35 during the late storage period. The data of hemolysis rate were grouped according to the sex and age of blood donors. Results The mean hemolysis rate during the late storage period was 0.20%, among which the hemolysis rate of the male group was higher than that of the female group, and the hemolysis rate of the 18-30 year old group was lower than that of the 31~40 year old group and that of the ≥41 years old group, respectively, with significant differences between the groups. Logistic regression equation showed that compared with the female group, the male group had an increased risk of hemolysis; and the 30~40 years old group and ≥41 years old group reported a higher risk of hemolysis than the 18~30 years old group, the difference being statistically significant. Conclusion Our findings suggest that the sex and age of blood donors are among the important factors affecting hemolysis rate, which could provide an accurate and detailed reference range for clinical quality control.

Key words: Red blood cells in additive solution, Hemolysis rate at the end of storage, Gender, Age