Analysis on Alleles of ABO Subgroups in Shandong Province

doi:10.3969/j.issn.1671-2587.2024.04.009

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2024, Vol. 26 ›› Issue (4): 479-483.DOI: 10.3969/j.issn.1671-2587.2024.04.009

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Analysis on Alleles of ABO Subgroups in Shandong Province

FENG Zhihui¹, BU Xiangmao², HUN Shouyong³, YU Shuhong⁴, ZHUANG Wenhua⁵, SU Jun⁶, ZHANG Wei⁷, GENG Wei⁸

¹Qingdao Blood Center, Institute of Transfusion Medicine, Qingdao;
²Qingdao Women and Children's Hospital, Clinical Laboratory, Qingdao;
³Shandong Provincial Hospital, Blood Transfusion Department, Jinan;
⁴Yantai Yu Huang Ding Hospital, Blood Transfusion Department, Yantai;
⁵Rizhao Blood Center, Hematology and Immunology Department, Rizhao;
⁶Weifang Blood Center, Institute of Transfusion Medicine, Weifang;
⁷Binzhou Blood Center, Quality Management Department, Binzhou;
⁸Jining Blood Center, Blood Distribution Department, Jining

Received:2024-05-27 Online:2024-08-20 Published:2024-09-23

Abstract

Abstract: Objectives Serology, ABO gene sequencing, and haplotype sequencing were performed on samples sent to our laboratory by medical institutions and blood stations in Shandong Province for suspected ABO subgroups. The distribution and molecular mechanism of ABO subgroup alleles in the population of Shandong Province were elucidated. Methods 239 blood donors suspected to be ABO subtypes and 701 clinical patients were tested for ABO gene sequence using PCR SBT method; Using monoclonal sequencing method to detect haplotypes; The software analyzes and compares the sequencing results to determine the allele subgroup of the sample. Results Molecular sequencing revealed 12 alleles of the A gene subgroup in Shandong Province, with ABO*A2.05 being the most common; There are 15 alleles in the B gene subgroup, with ABO*BW.03 being the most common; There are 6 types of complex ABO glycosyltransferase genes, with ABO*BA.04 being the most common; 6 cases of chimeric blood type. Conclusion ABO*A2.05 and ABO*A3.07 are the most common subgroups of the A gene in Shandong Province; ABO*BW.03 and ABO*BW.27 are the most common subgroups of the B gene; The ABO*BA.04 and ABO*cisAB.01 alleles are common complex ABO glycosyltransferase alleles.

Key words: ABO subgroups, A subgroups, B subgroups, ABO Glycosyltransferase with Dual Specificity Sequencing

CLC Number:

R457

FENG Zhihui, BU Xiangmao, HUN Shouyong, YU Shuhong, ZHUANG Wenhua, SU Jun, ZHANG Wei, GENG Wei. Analysis on Alleles of ABO Subgroups in Shandong Province[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(4): 479-483.

References

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Analysis on Alleles of ABO Subgroups in Shandong Province

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