Abstract: Objective Observation on the effectiveness of video stimulation test in prevention of blood donation-related vasovagal reaction (DRVR) caused by anxiety. Methods Blood donors in mobile blood collection vehicle No. 1 and No. 2 between September and October 2022, whose pre-blood donation health examination met GB18467-2001 "Health examination Requirements for Blood donors", Hamilton Anxiety Scale (HAMA) score less than 14 points, Barrier-free communication and cooperation, were selected as the study objects. Blood donors were divided into the experimental group and the control group. Video stimulation test was conducted before blood collection in the experimental group, that is, VR glasses were used to watch the blood collection video for 1 minute and 22 seconds, focusing on the venipuncture, blood outflow and other procedures. After watching, blood donation will be stopped immediately if the donor has any symptoms of physical anxiety, blood pressure and/or heart rate that do not meet the requirements of "Health Examination Requirements for Blood Donors" and HAMA score ≥14 points. Those without the above conditions began blood collection. Blood was collected directly without video stimulation in the control group. The HAMA score, blood pressure and heart rate were compared before and after the video stimulation test between the blood donor interval and the stopping blood donor interval in the experimental group. The reasons for stopping blood donors were analyzed. The incidence of DRVR and the causes of DRVR were compared between the two groups. Results There was no significant difference in HAMA score between the blood donor interval (7.54 ±2.41) and the stopping blood donor interval (7.58±2.84) before the video stimulation test in the experimental group (P>0.05). After the video stimulation test, the HAMA score of the stopping blood donors (12.88±3.51) was higher than that of blood donors (7.61±2.55) (P>0.05). The changes of systolic blood pressure, diastolic blood pressure and heart rate (2.25± 2.37, 1.32±1.64, 1.74±0.26, respectively) in the donor interval before and after the video stimulation test in the experimental group were lower than those in the stopping donor interval (5.41±3.87, 3.64±2.56, 3.87±1.38) (all P>0.05). For the reasons of stopping blood donors in the experimental group, there were 25 cases with any physical anxiety symptoms, accounting for 100.00%, 9 cases with blood pressure and/or heart rate that did not meet the "Health Examination Requirements for Blood Donors", accounting for 36.00%, and 5 cases with HAMA score≥14 points, accounting for 20.00%. The incidence of DRVR in the experimental group (1.11%) was lower than that in the control group (2.50%) (P>0.05). The proportion of DRVR blood donors caused by anxiety in the experimental group (0.22%) was lower than that in the control group (1.19%) (P>0.05). Conclusion The application of video stimulation test can induce anxiety among people who are sensitive to anxiety during the blood collection process. We identify anxiety symptoms and stop blood donation to prevent DRVR caused by anxiety and the incidence of DRVR to effectively protect blood donors'safety.

Key words: Video stimulation test, Donation related vasovagal reaction, Blood donation