Experimental Study on the Effects of Smoking on Platelet Count and Activation Function

doi:10.3969/j.issn.1671-2587.2025.01.004

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2025, Vol. 27 ›› Issue (1): 29-33.DOI: 10.3969/j.issn.1671-2587.2025.01.004

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Experimental Study on the Effects of Smoking on Platelet Count and Activation Function

LIU Zhen, TANG Longhai, JIN Yiming, XU Zihao, WANG Mingyuan

Suzhou Blood center, Suzhou 215006

Received:2024-06-19 Published:2025-02-25

Abstract

Abstract: Objective To investigate the changes in platelet function among smokers and the underlying causes, as well as the potential molecular mechanisms that may induce atherosclerosis. Methods A total of 122 samples from first-time male platelet-apheresis donors at Suzhou Blood Center were collected, which were divided into an experimental group (smokers) and a control group (non-smokers) based on smoking history, with ensuing analyses on platelet parameters, platelet activation level, platelet function, reactive oxygen species (ROS) levels, and mitochondrial function. Results Compared with the control group, the mean platelet volume (MPV) and platelet distribution width (PDW) in smokers were significantly increased (P<0.05), while platelet count showed no significant difference (P>0.05). Smokers' platelets presented increased CD62P and αⅡbβ3 (P<0.05), but after thrombin activation, the expression of CD62P and αⅡbβ3 was significantly lower than that of the control group (P<0.05), and the subsequent release of PF4 was insufficient (P<0.05). Moreover, TEG test results in smokers indicated a significant decrease in the R value (P<0.05) and a significant increase in the MA value compared to non-smokers (P<0.05), with no significant difference in the K value (P>0.05). Importantly, the level of ROS in smokers' platelets was significantly elevated (P<0.05), and the expression of mitochondrial probes was significantly reduced (P<0.05). Conclusion Smokers exhibit basal platelet activation, but upon thrombin activation, there is insufficient platelet activation and restricted release function, accompanied by elevated ROS production and compromised mitochondrial functionality.

Key words: Platelets, Platelet Activation, ROS, Mitochondria

CLC Number:

R457

LIU Zhen, TANG Longhai, JIN Yiming, XU Zihao, WANG Mingyuan. Experimental Study on the Effects of Smoking on Platelet Count and Activation Function[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(1): 29-33.

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Experimental Study on the Effects of Smoking on Platelet Count and Activation Function

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