Abstract: Objective To investigate the antiplatelet effect of tanshinone ⅡA (Tan IIA) and its inhibition effect of the interaction between human platelets and HCT116 cells of colon cancer. Method Whole blood or platelets of healthy volunteers were treated with different concentrations of Tan ⅡA (10, 20, 40 μmol/L), and inhibition rate of platelet aggregation triggered with Adenosine diphosphate (ADP) and arachidonic acid (arachidonic acid) were detected by TEG. The expression rates of CD62P and PAC-1 in platelets were detected by flow cytometry, the adhesion test was used to detect the adhesion of platelets to HCT116 cells after Tan ⅡA treatment, and the effect of platelets on the migration ability of HCT116 cells after Tan ⅡA treatment was tested by scratch test. Results The TEG results showed that Tan ⅡA inhibited ADP and AA-induced platelet aggregation in a concentration-dependent manner (P<0.01), low concentration Tan ⅡA treatment can obtain higher ADP inhibition rate, which is (73.48±19.63) %, high concentration Tan ⅡA treatment can obtain higher AA inhibition rate, which is (78.20±18.58) %. Flow cytometry detection showed that Tan IIA inhibited thrombin or ADP-induced expression of CD62P and PAC-1 on platelet surface in a concentration-dependent manner (P<0.05). Adhesion test results confirmed that Tan ⅡA could significantly inhibit the adhesion between thrombin or ADP-activated platelets and HCT116 cells, and the inhibition ability was positively correlated with Tan ⅡA concentration. The results of scratch test showed that activated platelets could promote the migration of HCT116 cells, and treatment of platelets with low concentration (10 μmol/L) Tan ⅡA could significantly inhibit promotion of platelet to the migration of tumor cells. Conclusion Tan IIA can inhibit the interaction between platelets and tumor cells by inhibiting platelet aggregation and activation.

Key words: Tanshinone ⅡA, Platelets, Bowel cancer, Activation, Transfer