Abstract: Objective To investigate the expression changes of long noncoding RNA (lncRNA) small nucleolar RNA host gene 9 (SNHG9) with extended storage during apheresis platelet storage. Methods A total of 32 samples of apheresis platelets that met the indications for platelet transfusion were collected and stored at （22±2）℃ with gentle agitation. Platelets were measured by appearance, platelet count, red blood cell residual count, white blood cell residual count, pH value and bacterial culture in different storage times (1, 3, 5, 7, and 9 days). The expression levels of lncRNA SNHG9 in different storage times were detected by quantitative real-time PCR (qRT-PCR) and the expressions of SNHG9 on platelet storage lesion (PSL) were analyzed. Results The expressions of SNHG9 with extended storage at the 1st, 3rd, 5th, 7th and 9th days were gradually increased. There was a statistical difference (H=93.073, P<0.001), with a statistical significance (P<0.05). SNHG9 expressions at different days were further compared by pairwise multiple comparisons. The corrected P value at the 1st and 3rd day was 0.144 with no statistical significance (P>0.05). The corrected P values at the 1st and 5th, 1st and 7th, 1st and 9th day were less than 0.001 with statistical significance (P<0.05). Statistically significant differences were corrected for on 3rd and 5th day (P=0.031), 3rd and 7th day (P<0.001), 3rd and 9th day (P<0.001). Conclusion With extended storage, SNHG9 expressions in apheresis platelets stored at the 5th, 7th, and 9th day were significantly higher than that at the 1st day. SNHG9 is sensitive to platelet storage condition, and may be a potential biomarker for PSL.

Key words: Apheresis platelets, LncRNA, SNHG9, Platelet storage lesion