• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2024, Vol. 26 ›› Issue (6): 750-754.DOI: 10.3969/j.issn.1671-2587.2024.06.006

Previous Articles     Next Articles

Analysis and Clinical Significance of Minor Cross-match Incompatibility with Micro-column Gel Test

WANG Danting1,2, LONG Honghui1,2, NIU Yingying1,2, HUANG Chunyan1,2   

  1. 1Department of Transfusion Medicine, West China Hospital, Sichuan University, Chengdu 610041;
    2Department of Transfusion Medicine, West China Tianfu Hospital, Sichuan University, Chengdu 610213
  • Received:2024-06-26 Online:2024-12-20 Published:2024-12-20

Abstract: Objective To analyze the cause and significance of minor cross-match incompatibility by using micro-column gel test (MGT), and to provide reference for clinical transfusion. Methods Samples of transfusion recipients were collected from July 2022 to July 2023 with minor cross-match incompatible while antibody screening test negative. Direct antiglobulin test (DAT) was performed, Positive samples undergoing acid elution test. Antibody screening and identification were conducted on the eluate. DAT-negative individuals underwent donor antibody screening or re-cross-matching. A part of DAT-positive transfused patients were selected as the experimental group, and patients with major and minor cross-match compatibility and negative DAT were selected by propensity score matching as the control group for comparison and evaluation of transfusion efficacy. Results Among 380 patients with major cross-match compatibility and the minor cross-match incompatibility, 372 patients were DAT positive (97.89%). Antibody test of the acid elute, 364 cases showing negative results (97.85%), 6 cases were positive (1.61%) with no pattern. Two cases showed suspected reactive patterns (0.54%), but no specific antibodies were identified. These patients exhibited a significant increase in hemoglobin after receiving red blood cell suspension transfusion (P<0.05), with no impact on the safety and efficacy of transfusion compared to the control group. Among DAT-negative patients, 4 cases were antibody screening positive (1.05%), 1 case had gel card abnormalities (0.26%), 2 cases were due to human error (0.52%), and 1 case was a false aggregation (0.26%). Conclusion The minor cross-match incompatibility with MGT has almost no impact on the safety and efficacy of transfusion for patients, and the majority of reasons for minor cross-match incompatibility are due to DAT positivity in patients. In addition, a small number of minor cross-match incompatibility were caused by human error, false aggregation and abnormal gel cards.

Key words: Cross-match, Minor incompatibility, Direct antiglobulin test, Blood transfusion efficacy

CLC Number: