Abstract: Objective To evaluate and analyse the testing result of HTLV-Ⅰ/Ⅱ infection in Shijiazhuang district in order to provide a scientific basis for clinical blood transfusion safety in this area. Methods Antibody of HTLV-Ⅰ/Ⅱ was measured with double antigen sandwich enzyme-linked immunosorbent assay （ELISA）kit from Sep to Dec in 2016 in Hebei blood center. The positive blood samples in the first test were re-tested by double-hole retrial method．Then the sero-positive samples were confirmed by RT-PCR or Western blot in National Center for Clinical Laboratories. Results Among 41 000 donors in Shijiazhuang district,25 cases were detected positive in the first test but only one case were confirmed positive with RT-PCR or WB. Retrospective investigation revealed that this HTLV sero-positive donor had had the eligible records as high as four blood donations in Hebei blood center. Conclusions These results indicate that Shijiazhuang is non-endemic for HTLV-I as well as HTLV-II. ELISA method can be used for HTLV-Ⅰ/Ⅱscreening to epidemiological investigation,due to the low rate of false positives.

Key words: HTLV-Ⅰ/Ⅱ, Voluntary blood donors, Blood screening