Abstract: Objective The feasibility of returning to the team was discussed through two follow-up and analysis of single nucleic acid testing（NAT）positive blood donors. Methods The single NAT positive blood donors contacted by telephone were followed up for two consecutive times after informed consent. The samples were followed up for routine detection of 3 items of ELISA,3 items of NAT（detected by 2~3 nucleic acid systems at the same time）and 5 items of HBV by chemiluminescence. The results were analyzed statistically. Results 28 donors were followed twice. 21 NAT tested positive at least once（75%）; 27 HBcAb tested positive（96.43%）; 16 HBsAb tested positive （76.19%）. Conclusion The single NAT positive blood donors are mainly infected by HBV occult infection, and the risk of returning to blood donation is large, so it is necessary to establish the returning route carefully.

Key words: HBV, Blood donors, NAT