一种显微计数新方法ADAM-rWBC在成分血残留白细胞检测中的应用

doi:10.3969/j.issn.1671-2587.2016.01.016

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (1): 51-56.DOI: 10.3969/j.issn.1671-2587.2016.01.016

一种显微计数新方法ADAM-rWBC在成分血残留白细胞检测中的应用

曾晓晖,张雅丽,洪素云,谢蓓蓓,曾晓新,倪宏英,宋秀宇,陈长荣

361004 福建省厦门市中心血站

收稿日期:2015-08-23 出版日期:2016-02-20 发布日期:2016-10-10
通讯作者: 陈长荣
作者简介:曾晓晖（1973-）,男,福建厦门人,副主任药师,学士,主要从事血液管理控制研究,E-mail:strongwall@163.com。

Detection of the Residual Leukocytes in Blood Components: a Method of ADAM-rWBC for Microscopic Counting

ZENG Xiao-hui, ZHANG Ya-li, HONG Su-yun, et al

Xiamen Blood Center of Fujian Province 361004

Received:2015-08-23 Online:2016-02-20 Published:2016-10-10

摘要/Abstract

摘要： 目的评估一种基于显微计数新方法（ADAM-rWBC）在残余白细胞定量检测中的性能和应用可行性。方法以Sysmex KX21全自动血球计数法及Nageotte血细胞计数板法作为参比,分析ADAM-rWBC检测白细胞的定量准确性和可重复性,并评估其在成分血白细胞残留检测中的实际性能。结果对WBC浓度分别为40、10和1 cell/µl的3份滤白红细胞悬液样品进行平行检测,ADAM-rWBC的批间变异系数分别为8.8、11.3和31.1,准确度分别为100.0%、87.5%和37.5%;而Nageotte计数板法的批间变异系数则分别为20.4、31.8和15.3,准确度分别为50.0%、12.5%和37.5%。对36份全血标本（白细胞浓度范围为4辱与共4 500~10 700 cell/µl）,采用ADAM-rWBC进行稀释定量与采用Sysmex kx21进行直接计数的结果间差异无统计学意义（P=0.655）,且线性相关性较好（Slope=1.00±0.01, R2=0.832,P<0.001）。对34份滤白红细胞悬液进行残留白细胞测定,平行采用ADAM-rWBC和Nageotte计数法所获结果间的差异无统计学意义（P=0.18）,两种方法定量值均低于3.0 cell/µl,符合白细胞残余量<12.5 cell/µl的质控要求。结论采用ADAM-rWBC进行白细胞定量测定准确可靠,相比于Nageotte计数法重复性和精度更佳,能满足成分血白细胞残余量检测的国标需求。

关键词: 成分血质控, 白细胞残余检测, 显微计数新方法

Abstract: ObjectiveTransfusion of blood components containing high amounts of leucocytes possibly causes side-effects in recipients. Therefore, the measurement of the residual leucocytes is certainly required for quality control of blood components. The study aimed to evaluate the performance of a new microscopic system (ADAM-rWBC) for residual leucocytes determination in blood components. MethodsThe quantitative accuracy, reproducibility and practical performance of ADAM-rWBC in detection of leucocytes was evaluated when compared with sysmex KX21 hematology analyzer and manual counting in the nageotte chamber. ResultsIn a parallel detection of 3 specimens which contained 40, 10 and 1 cell/μl, the inter-assay coefficient of variation (CV %) was 8.8, 11.3, and 31.1 for ADAM-rWBC and 20.4, 31.8, and 15.3 for Nageotte. The accuracy was 100%, 87.5%, and 37.5% for ADAM-rWBC and 50%, 12.5%, and 37.5% for Nageotte, respectively. In the detection of 36 complete blood samples (white leucocytes counting ranged from 4500 to 10 700 cell/μl), the WBC quantification results from ADAM-rWBC diluting- measurement were comparable (P =0.655) and well-correlated (Slope=1.00±0.01, R 2=0.832, P <0.001) with that from Sysmex kx21 hematology analyzer. In detection of 34 blood components after leucocytes depletion, no significant difference (P =0.18) was observed between ADAM-rWBC and Nageotte chamber, all sample presenting a WBC amount <3.0 cell/ μl, which coincided with the standard requirement of <12.5 cell/μl. ConclusionThe ADAM-rWBC was a accurate and reliable method for white leucocyte measurement. In comparison with manual counting, the ADAM-rWBC was more reproducible and was satisfied of convenience and be able to meet the national requrement.

中图分类号:

R331.1+42

曾晓晖,张雅丽,洪素云,谢蓓蓓,曾晓新,倪宏英,宋秀宇,陈长荣. 一种显微计数新方法ADAM-rWBC在成分血残留白细胞检测中的应用[J]. 临床输血与检验, 2016, 18(1): 51-56.

ZENG Xiao-hui, ZHANG Ya-li, HONG Su-yun, et al. Detection of the Residual Leukocytes in Blood Components: a Method of ADAM-rWBC for Microscopic Counting[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2016, 18(1): 51-56.

参考文献

[1] Vamvakas EC, Blajchman MA. Universal white-cell reduction: the case for and against[J]. Transfusion, 2001,41(5):691.
[2] Vamvakas EC. The case against universal white blood cell reduction[J]. ISBT Science Series,2006, 1(1):64.
[3] Dzik S,Aubuchon J,Jeffries L,et al. Leukocyte reduction of blood components: public policy and new technology[J]. Transfusion Medicine Review, 2000,14(1):34.
[4] Centers for Disease Control and Prevention (CDC). Adverse ocular reactions following transfusions United States 1997-1998[J]. MMWR Morb Mortal Wkly Rep,1998,47(3):49.
[5] Lutz P, Dzik WH.Large-volume hemocytometer chamber for accurate counting of white cells (WBCs) in WBC-reduced platelets: validation and application for quality control of WBC-reduced platelets prepared by apheresis and filtration[J]. Transfusion, 1993, 33(5):409.
[6] Borzini P, Dumont LJ. Microdroplet fluorochromatic assay for the enumeration of white cells (WBCs) in WBC-reduced blood components: validation and application for evaluating newly developed WBC-reduction filters[J]. Transfusion,1997,37(6):601.
[7] 沈卓岚,徐健,徐芳. 血小板成分中残留白细胞的新检测方法[J]. 中国输血杂志,2007,20(3):206.
[8] Wang YJ, Sheng ZL, Xu J, et al. Evaluation of two methods for counting residual white blood cells in thrombocytaphoresis concentrates[J]. Journal of Experimental Hematology,2006,14(3):614.
[9] Palmer DS,Birch P,O&apos;Toole J,et al. Flow cytometric determination of residual white blood cell levels in preserved samples from leukoreduced blood products[J]. Transfusion,2008,48(1):118.
[10] Bae SY,Lee CH,Kim JS,et al. Portable microscopic cell counter for the determination of residual leucocytes in blood components[J]. Vox Sang,2007, 92(1):64.
[11] Strobel J,Antos U,Zimmermann R,et al. Comparison of a new microscopic system for the measurement of residual leucocytes in apheresis platelets with flow cytometry and manual counting[J].Vox Sang,2014,107(3):233.
[12] Kim JS,Nam MH,An SS,et al. Comparison of the automated fluorescence microscopic viability test with the conventional and flow cytometry methods[J]. J Clin Lab Anal,2011,25(2):90.
[13] GB 18469-2001. 全血及成分血质量要求[S].

一种显微计数新方法ADAM-rWBC在成分血残留白细胞检测中的应用

Detection of the Residual Leukocytes in Blood Components: a Method of ADAM-rWBC for Microscopic Counting

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