Abstract: ObjectiveTo evaluate the detection methodology of urinary retinol binding protein(RBP) by latex immune-nephelometry. MethodsTyhe Precision, linear range, accuracy, sensitivity, and specificity of the examination methodology that is currently used were evaluated according to the standard protocol of National Committee for Clinical Laboratory Standards (CLSI) documents. ResultsThe lowest detection limit in this methodology was 0.037 8 mg/L and the linear range was 0~10 mg/L. Repeatability precision was 1.121%, inter-assay precision was 5.536% and reproducibility precision was 6.657%. The average recovery rate was 101.83% and there was no interference to RBP detection under the existence of <100 mg/L total bilirubin or <10 mg/L hemoglobin. There was a positive correlation between the present methodology and the control reagent of urinary RBP detection (r2 =0.974 0), which was based on the platform of automatic biochemical analyzer, and there was no significance comparing with the results of urinary RBP of 40 clinical samples using these two methodology. ConclusionThe present method of urinary RBP detection, with high sensitivity, precision, accuracy and strong anti-interference, is suitable for clinical laboratory point-of-care testing (POCT).