曲古菌素A联合全反式维甲酸诱导卵巢癌SK0V-3细胞株凋亡的研究

doi:10.3969/j.issn.1671-2587.2017.04.003

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (4): 321-324.DOI: 10.3969/j.issn.1671-2587.2017.04.003

曲古菌素A联合全反式维甲酸诱导卵巢癌SK0V-3细胞株凋亡的研究

盛佳佳, 刘雨生

230001 合肥,安徽医科大学附属妇幼保健院,安徽省妇幼保健院

收稿日期:2017-01-28 出版日期:2017-08-20 发布日期:2017-08-09
通讯作者: 刘雨生,男,主任医师,主要从事生殖医学与内分泌学研究,（E-mail）shengzhizhongxin@126.com。
作者简介:盛佳佳（1985-）,女,安徽合肥人,医师,硕士,主要从事妇科肿瘤及生殖医学研究工作,（E-mail）sheng13956942890@163.com。

Trichostatin A Combined with All-trans Retinoic Acid Induce Apoptosis in Human Ovarian Cancer Cell Line SKOV-3 SHENG

Jia-jia, LIU Yu-sheng

Anhui Women and Child Health Care Hospital,HeFei,230001

Received:2017-01-28 Online:2017-08-20 Published:2017-08-09

摘要/Abstract

摘要： 目的探讨TSA联合ATRA对卵巢癌SKOV-3细胞的协同增殖抑制和凋亡作用。方法 TSA联合 ATRA处理卵巢癌细胞SKOV-3细胞株,分别检测SK0V-3细胞增殖抑制情况和细胞凋亡率及相关蛋白表达变化。结果 TSA联合ATRA协同抑制SKOV-3细胞生长,差异有统计学意义（P<0.05）;联合组SKOV-3细胞凋亡率明显升高,与对照组和单独用药组比较,差异有统计学意义（P<0.05）。结论 TSA联合ATRA在体外对卵巢癌SKOV-3细胞具有较强的协同增殖抑制和促进凋亡作用。

关键词: 曲古菌素A, 全反式维甲酸, 细胞凋亡, SKOV-3细胞

Abstract: Objective To investigate the apoptotic effect on human ovarian cancer cells by both Trichostatin A（TSA）and all-trans retinoic acid（ATRA）and synergistic effect. Methods SKOV-3 cells were treated by different group of TSA and ATRA. The inhibitory rate of proliferation and the apoptosis rate were examined. Western blot was introduced to evaluate the protein expression levels. Results The proliferation of SKOV-3 cells was significantly suppressed by TSA combined with ATRA and the difference was significant compared with other groups （P<0.05）. The apoptosis rate of combination group was higher than that of other groups and the difference was significant （P<0.05）. Conclusion TSA combination with ATRA induces cytotoxicity in ovarian cancer cells in vitro,and the effect was much more significant than other groups.

Key words: Trichostatin A, All-trans retinoic acid, Cell apoptosis, SKOV-3 cells

中图分类号:

R984R737.31

盛佳佳, 刘雨生. 曲古菌素A联合全反式维甲酸诱导卵巢癌SK0V-3细胞株凋亡的研究[J]. 临床输血与检验, 2017, 19(4): 321-324.

Jia-jia, LIU Yu-sheng. Trichostatin A Combined with All-trans Retinoic Acid Induce Apoptosis in Human Ovarian Cancer Cell Line SKOV-3 SHENG[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2017, 19(4): 321-324.

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曲古菌素A联合全反式维甲酸诱导卵巢癌SK0V-3细胞株凋亡的研究

Trichostatin A Combined with All-trans Retinoic Acid Induce Apoptosis in Human Ovarian Cancer Cell Line SKOV-3 SHENG

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