• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (4): 358-361.DOI: 10.3969/j.issn.1671-2587.2017.04.014

• 临床输血 • 上一篇    下一篇

丙型肝炎病毒抗体复查策略的研究

张菁, 邵春燕, 卓海龙, 王海平, 骆群   

  1. 100071 中国人民解放军第三〇七医院输血科
  • 收稿日期:2016-12-16 出版日期:2017-08-20 发布日期:2017-08-09
  • 通讯作者: 骆群（1967-）,男,硕士生导师,副主任医师,主要从事临床输血研究,（Tel）010-66947206（E-mail）luoq66@aliyun.com。
  • 作者简介:张菁（1987-）,女,蒙古族,内蒙古呼和浩特人,技师,本科,主要从事血液检验工作,（Tel）15901132614（E-mail）465933707@qq.com。

Study on the Rechecked Strategy of Antibody to Hepatitis C Virus

ZHANG Jing, SHAO Chun-yan, ZHUO Hai-long, et al   

  1. The 307th Hospital of Chinese People's Liberation Army 100071
  • Received:2016-12-16 Online:2017-08-20 Published:2017-08-09

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摘要： 目的 探讨化学发光法（chemiluminescence immunoassay,CLIA）联合酶联免疫法（enzyme linked immunosorbent assay,ELISA）检测丙型肝炎病毒抗体（抗-HCV）的复查策略。方法 采用CLIA法筛选出抗-HCV S/CO﹥0.40的血清样本,再采用2种ELISA法试剂进行复查,对至少1种试剂检测结果在临界值（CLIA法S/CO﹥0.90,ELISA法S/CO﹥0.80）以上的样本,采用荧光定量聚合酶链反应（realtime fluorescence quantitative PCR,FQ-PCR）方法进行HCV核酸定量（HCV RNA）。结果 从本院临床患者中筛选出CLIA法抗-HCV S/CO﹥0.40的样本进行ELISA法检测,至少1种试剂检测结果在临界值以上的血清样本201例,其中强生CLIA、索林ELISA和科华ELISA法阳性率分别为2.5％（150/5 800）、1.6%（96/5 800）和1.5%（89/5 800）。CLIA及2种ELISA法试剂检测结果均呈阳性的样本75例,阳性率为1.3%（75/5 800）;FQ-PCR法检测结果阳性51例,阳性率为0.9%（51/5 800）。CLIA法检测抗-HCV S/CO﹥0.40且<0.90的样本有40份,CLIA法检测抗-HCV S/CO﹥0.90且<0.99的样本有8份;CLIA法检测抗-HCV S/CO≥1.0的样本有153例,其中S/CO 1.00~5.00的样本有66例,S/CO 5.01~10.00的样本16例,S/CO 10.01~20.00的样本5例,S/CO﹥20.00的样本66例。结论 对CLIA法检测抗-HCV S/CO﹥0.40且<0.90的样本未发现漏检,建议直接发放阴性结果,减少试剂浪费;对CLIA和ELISA法检测抗-HCV阳性样本进行HCV RNA检测,并结合临床表现进行诊断及疗效评价。

关键词: 化学发光法, 酶联免疫法, 丙型肝炎病毒抗体

Abstract: Objective To Investigate the rechecked strategy of chemiluminescence immunoassay（CLIA） combined with enzyme linked immunosorbent assay（ELISA）in order to detect Hepatitis C virus antibodies. Methods Two hundred and one sera samples of CLIA anti-HCV S/CO﹥0.40 were collected from the patients by ELISA. and the samples over the cutoff value with at least one reagent detection were checked for HCV RNA by realtime fluorescence quantitative PCR（FQ-PCR） . Results Among the positive 201 samples,CLIA provided by Johnson,ELISA by MUREX,and ELISA by Kehua companies revealed positivities of 2.5％,1.6%,and 1.5%,respectively. There were 75 （1.3%,75/5 800）samples that had both CLIA and two ELISA reagents positive detections. FQ-PCR positive detections were noted in 51（0.9%,51/5 800）samples. Forty samples exhibited anti-HCV S/CO﹥0.40 and <0.90 in CLIA detection. Eight samples showed anti-HCVS/CO﹥0.90 and <0.99 in CLIA detection. There were 153 samples that revealed the anti-HCV S/CO≥1.0 in CLIA detection,among them,66 samples indicated S/CO 1.00-5.00,16 samples,S/CO 5.01-10.00; 5 samples,S/CO 10.01-20.00; and 66 samples,S/CO﹥20.00. Conclusion CLIA is reliable for detection of anti- HCV with S/CO﹥0.40 and <0.90. HCV RNA detection is useful for laboratory diagnosis and treatment evaluation in the samples that have showed positive anti-HCV by using CLIA and ELISA.

Key words: Chemiluminescence immunoassay, Enzyme linked immunosorbent assay, Hepatitis C virus antibody

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