• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (5): 430-433.DOI: 10.3969/j.issn.1671-2587.2017.05.005

• 临床输血 • 上一篇    下一篇

短期快速失血对机体脂质过氧化水平影响的实验研究*

李大鹏, 吕春雷, 徐会斌, 郑文哲, 王凤娇, 张霞   

  1. 271000 山东省泰安市解放军第88医院中心实验室
  • 收稿日期:2017-06-02 出版日期:2017-10-20 发布日期:2017-10-26
  • 通讯作者: 吕春雷,男,主任医师,（E-mail）15653808901@163.com。
  • 作者简介:李大鹏（1964-）,男,辽宁锦州人,副主任技师,主要从事医学实验工作,（E-mail）lidapeng1964@126.com。
  • 基金资助:
    *本课题受军队“十二五”后勤科研计划项目（No.CWS11J297）资助

Experimental Study on the Change of Organism Lipid Peroxidation Level in Short Term Rapid Blood loss

LI Da-peng, LV Chun-lei, XU Hui-bin, et al   

  1. The 88th Hospital of PLA,Taian 271000
  • Received:2017-06-02 Online:2017-10-20 Published:2017-10-26

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摘要： 目的 探讨短期快速失血对机体脂质过氧化水平的影响。方法 选择普通级新西兰兔11只,通过急性失血方式建立收缩压下降曲线,形成快速失血模型,测定收缩压基础值（T1）,当收缩压降至基础值2/3（T2）、基础值1/2（T3）、基础值1/3（T4）时采集血样,采用酶联免疫吸附分析检测各位置血清丙二醛（malondialdehyde MDA）、过氧化氢酶（hydrogen peroxide enzyme CAT）、谷胱甘肽过氧化物酶（glutathione oxidation reductase GSH-PX）活性,并予以统计分析。结果 MDA ：B组低于C组、C组低于D组,各时间水平间差异无统计学意义（P>0.05）;CAT ：A组高于B组、B组高于C组、C组高于D组,T1-T2、T2-T3、T3-T4各组间差异无统计学意义（P>0.05）,其余组间差异存在统计学意义（P<0.05）;GSH-Px ：A组高于B组、B组高于C组、C组高于D组,T1、T2间差异无统计学意义（P>0.05）,其余组间差异存在统计学意义（P<0.05）。结论 短期快速失血对机体抗氧化酶CAT、GSH-Px具有明显影响,可致CAT、GSH-Px活性下降,而反映脂质过氧化程度的MDA水平则上升,失血量与CAT、GSH-Px指标间相关性具有统计学意义。

关键词: 失血, 脂质过氧化, 丙二醛, 抗氧化酶, 过氧化氢酶, 谷胱甘肽过氧化物酶, 抗氧化能力

Abstract: Objective Study on the changes of organism lipid peroxidation level in short term rapid blood loss. Methods The rapid blood loss model of the gradient decerease in the systolic blood pressure was eatablished in 11 new zealand rabbits on ordinary level. The arterial blood during the different phase of shock,i.e.when the systolic pressures were 2/3（T2）,1/2（T3）,1/3（T4）of the basis systolic blood pressure,was collected and malondialdehyde（MDA）、hydrogen peroxide enzyme （CAT）、glutathione oxidation reductase （GSH-P x）levels in the plasma were determined by the double antibody sandwich method of enzyme-labeled immunosorbent assay（ELISH）,and the data be statistically analyzed. Results MDA ：Group B less than in group C,group C less than in group D,there was no significant difference between the time level（P>0.05）. CAT ：Group A higher than in group B,group B higher than in group C,group C higher than in group D,there was no significant difference between the T1-T2,T2-T3,T3-T4（P>0.05）,there were significant differences between the other groups （P<0.05）. GSH-Px ：Group A higher than in group B,group B higher than in group C,group C higher than in group D,there was no significant difference between the T1-T2（P>0.05）,there were significant differences between the other groups （P<0.05）. Conclusion Short term rapid blood loss has a significant effect on the antioxidant enzyme CAT and GSH-Px of organism,can lead to significantly decrease in CAT and GSH-Px activity,MDA levels rise reflect the degree of lipid oxidation. There were significantly correlated between blood loss and CAT,GSH-Px index.

Key words: Lose, blood, Lipid, peroxidation, Malondialdehyde, Antioxidant, enzyme, Hydrogen, peroxide, enzyme, Glutathione, oxidation, reductase, Antioxidant, capacity

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