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临床输血与检验

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (5): 412-415.DOI: 10.3969/j.issn.1671-2587.2016.05.002

• 基础研究 • 上一篇    下一篇

外源性谷氨酰胺对机体抗氧化酶影响的实验研究

李大鹏, 翟真真, 张霞, 王凤娇, 郑文哲   

  1. 271000 山东省泰安市解放军第88医院（李大鹏,翟真真,张霞,王凤娇）; 医务处（郑文哲）
  • 收稿日期:2016-01-25 出版日期:2016-10-20 发布日期:2017-05-19
  • 通讯作者: 翟真真,女,硕士,（Tel）13605383048（E-mail）718568624@qq.com。
  • 作者简介:李大鹏（1964-）,男,辽宁锦州人,副主任技师,（Tel）13583848186（E-mail）lidapeng1964@126.com。

An Experimental Study of Exogenous Glutamine Influence on the Antioxidant Enzymes

LI Da-peng, ZHAI Zhen-zhen, ZHANG Xia, et al   

  1. The 88th Hospital of PLA,Taian 271000
  • Received:2016-01-25 Online:2016-10-20 Published:2017-05-19

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摘要： 目的 探讨外源性谷氨酰胺（Gln）对机体抗氧化酶的影响。方法 选择无特定病原体成年大鼠22只,随机分为实验组（A）、对照组（B）、标准组（C）,A组每日丙胺酰谷氨酰胺注射液灌胃,B组0.9%生理盐水灌胃,C组不灌胃;A、B两组灌服时间均为连续14 d,完毕后留取血样检测3组大鼠血清过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性,并予以统计分析。结果 CAT ：A、C组间差异有统计学意义（P <0.05）,其余组间差异无统计学意义（P >0.05）,实验组14 d时血清CAT水平显著高于标准组（P <0.05）;GSH-Px ：A、C组间差异有统计学意义（P <0.05）,A与B组间差异无统计学意义（P >0.05）,实验组14 d时血清GSH-Px水平显著高于标准组（P <0.05）;SOD :各组间差异均无统计学意义（P >0.05）。结论 短期外源性补充Gln可显著提高血清CAT及GSH-Px活性水平,并可提高血清SOD活性水平,提高机体清除自由基及抗氧化能力。

关键词: 脂质过氧化, 自由基, 抗氧化酶, 过氧化氢酶, 超氧化物歧化酶, 谷胱甘肽过氧化物酶, 谷氨酰胺

Abstract: Objective To explore the influence of exogenous glutamine（Gln）on the antioxidant enzymes. Methods Twenty two rats (specific pathogen free) were randomly divided into experimental group（A）,control group（B）,and standard group（C）. Animals of group A received gavage daily with alanyl-glutamine,group B with 0.9% normal saline,and group C were set up as control. Groups A and B were given gavage consecutively for 14 days. Blood specimens of rats of the three groups were detected for hydrogen peroxide enzyme（CAT）, superoxide dismutase（SOD）and glutathione oxidation reductase（GSH-Px）. Results The difference of CAT between groups A and C was statistically significant（P<0.05）, while the level of CAT at 14d of experimental group was significantly higher than the standard group（P<0.05）. The difference of GSH-Px between groups A and C was statistically significant（P<0.05）, no difference between groups A and B was found（P>0.05）.The level of GSH-Px at 14d of experimental group was remarkably higher than that of standard group（P<0.05）. No significant difference of SOD was noted among all of the three groups（P>0.05）. Conclusion Temperal administration of exogenous Gln may significantly increase CAT and GSH-Px levels, and serum SOD as well,thereby enhancing the ability to scavenge free radicals and antioxidant of the body.

Key words: Lipid peroxidation, Free radicals, Antioxidant enzyme, Hydrogen peroxide enzyme, Superoxide dismutase, Glutathione oxidation reductase, Glutamine

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