碳青霉烯中介肠杆菌科细菌耐药性及相关基因检测

doi:10.3969/j.issn.1671-2587.2017.06.025

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (6): 614-617.DOI: 10.3969/j.issn.1671-2587.2017.06.025

碳青霉烯中介肠杆菌科细菌耐药性及相关基因检测

张霞, 朱超望, 王莹超, 眭阳, 尹娟

215008 苏州,南京医科大学附属苏州医院北区检验科

收稿日期:2017-03-17 出版日期:2017-12-20 发布日期:2017-12-13
作者简介:张霞（1975-）,女,江苏南通人,副主任技师,学士,主要从事临床检验工作,（Tel）13962164303（E-mail）616463097@qq.com。

Investigate on the Carbapenemases Genes and Drug Resistance in Carbapenem-intermediate Enterobacteriaceae

ZHANG Xia, ZHU Chao-wang, WANG Ying-chao, et al

Department of Clinical Laboratory,The North District of Affiliated Suzhou Hospital,Nanjing Medical University,Suzhou 215008

Received:2017-03-17 Online:2017-12-20 Published:2017-12-13

摘要/Abstract

摘要： 目的分析碳青霉烯类药物敏感性中介肠杆菌科细菌（Carbapenem-intermediate Enterobacteriaceae,CIE）的耐药特性及其携带碳青霉烯酶基因情况。方法收集2015年3月~2017年1月南京医科大学附属苏州医院北区分离的26株CIE,分析菌株对抗菌药物的敏感性,并通过聚合酶链反应（Polymerase Chain Reaction,PCR）筛选碳青霉烯酶基因KPC、NDM、IMP、VIM和OXA48基因。结果 26株临床分离的CIE菌株中,对氨苄西林、头孢唑林、头孢他啶等药物耐受均达到80%以上,PCR法检测到6株细菌携带碳青霉烯酶基因,包括KPC 4株、NDM 1株、IMP 1株。结论碳青霉烯中介肠杆菌科细菌多重耐药率较高,且检出碳青霉烯酶基因的菌株也占一定比例,需进一步加强临床多重耐药细菌的监测。

关键词: 肠杆菌科细菌, 多重耐药, 碳青霉烯酶基因

Abstract: Objective To investigate the drug resistance of Carbapenem-intermediate Enterobacteriaceae （CIE）,and whether carrying carbapenemases gene. Methods 26 CIE isolates were collected from March 2015 to January 2017 in the North District of Affiliated Suzhou Hospital,Nanjing Medical University,drug resistance of these isolates were investigated by antibiotic susceptibility testing. Carbapenemase genes KPC,NDM,IMP,VIM and OXA48 were detected by polymerase chain reaction （PCR）. Results More than 80% of 26 CIE clinical isolates were resistance to ampicillin,cefazolin,ceftazidime. PCR detected 6 isolates carrying carbapenemase genes,including 4 isolates of KPC,1 isolate of NDM and 1 isolate was IMP positive. Conclusion The multidrug resistance rate in carbapenems-intermediate Enterobacteriaceae was high,and the carbapenemases gene positive isolates were also account for a certain proportion,which suggests that the clinical monitoring of multiple drug-resistant bacteria needs to be further strengthen.

Key words: Enterobacteriaceae, Multidrug resistance, Carbapenemases gene

中图分类号:

R378.2R392.11

张霞, 朱超望, 王莹超, 眭阳, 尹娟. 碳青霉烯中介肠杆菌科细菌耐药性及相关基因检测[J]. 临床输血与检验, 2017, 19(6): 614-617.

ZHANG Xia, ZHU Chao-wang, WANG Ying-chao, et al. Investigate on the Carbapenemases Genes and Drug Resistance in Carbapenem-intermediate Enterobacteriaceae[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2017, 19(6): 614-617.

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碳青霉烯中介肠杆菌科细菌耐药性及相关基因检测

Investigate on the Carbapenemases Genes and Drug Resistance in Carbapenem-intermediate Enterobacteriaceae

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