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临床输血与检验

临床输血与检验 ›› 2018, Vol. 20 ›› Issue (4): 428-430.DOI: 10.3969/j.issn.1671-2587.2018.04.027

• 临床检验 • 上一篇    下一篇

血清高尔基体糖蛋白73在慢性肝病诊断中的应用研究

王钢,陈涛,卢峰,李甫罡,郭晓兰,罗志刚   

  1. 641400 四川省简阳市人民医院中心实验室
  • 收稿日期:2017-12-23 出版日期:2018-08-20 发布日期:2018-08-14
  • 通讯作者: 罗志刚,男,四川资阳人,主任医师,博士,（Tel）028-27238249。
  • 作者简介:王钢（1985–）,男,四川广元人,硕士,主要从事细胞与分子生物学工作,（Tel）18708219836,（E-mail）314775289@qq.com。

Application Study of Serum Golgi Glycoprotein 73 in Diagnosis of Chronic Liver Diseases

WANG Gang,CHEN Tao,LU Feng,et al.   

  1. Central Laboratory,People's Hospital of Jianyang,Jianyang 641400
  • Received:2017-12-23 Online:2018-08-20 Published:2018-08-14

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摘要： 目的 探讨高尔基体蛋白73（GP73）在慢性肝病诊断中的临床应用价值。方法 收集本院原发性肝癌（HCC）（91例）、乙肝后肝硬化（217例）、慢性乙型病毒性肝炎患者（230例）和健康人群（62例）血清，采用酶联免疫吸附测定法（ELISA）和电化学发光法分别检测血清GP73、AFP含量，并对其特异性和敏感性进行统计分析。结果 HCC患者血清GP73最高，其次分别为肝硬化、慢性乙型肝炎患者，健康人群不升高，两两比较差异均具有统计学意义（P＜0.05）。GP73的敏感性和特异性（分别为65.9%、79.5%）与AFP的敏感性和特异性（分别为61.5%、74.6%）基本一致，但AFP与GP73联合检测可明显提高诊断的敏感性和特异性（84.6%和90.1%）；并且HCC患者血清GP73含量与其TBIL、DBIl、TBA、ALT、AST水平具有相关性。结论 GP73在肝癌筛查中敏感性和特异性均较高，且GP73与AFP联合检测可提高HCC诊断准确性与临床应用价值。

关键词: 原发性肝癌, 高尔基体蛋白73, 甲胎蛋白

Abstract: Objective To investigate the application value of Golgi glycoprotein 73（GP73）in the diagnosis of chronic liver diseases. Methods The peripheral serum samples were collected from patients with hepatocellular carcinoma （HCC）（n=91），hepatocirrhosis（n=217），hepatitis（n=230），and healthy control group（n=62），and the serum GP73 and AFP were determined by Elisa and electrochemiluminescence . Methods respectively. Then the specificity and sensitivity were statistically analyzed. Results The level of serum GP73 was the highest in patients with HCC，followed by hepatocirrhosis group，hepatitis group and healthy control group（P<0.05）. In the diagnosis of HCC，the sensitivity and specificity of GP73 was 65.9% and 79.5%，respectively，and the sensitivity and specificity of AFP for HCC were 61.5% and 74.6%，respectively. While combined measurement of AFP and GP73 increased the sensitivity and specificity to 84.6% and 90.1% respectively. Moreover，in patients with HCC，the level of AFP was correlated with serum DBIL and AST，while GP73 level was associated with TBIL，DBIL，TBA，ALT and AST. Conclusion The sensitivity and specificity of GP73 were high enough for liver cancer screening，while combination measurement of GP73 and AFP would further improve clinical application value in diagnosis of hepatocellular carcinoma.

Key words: Hepatocellular carcinoma , Golgi protein 73, Alpha fetal protein

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