• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2019, Vol. 21 ›› Issue (3): 320-322.DOI: 10.3969/j.issn.1671-2587.2019.03.026

• 临床检验 • 上一篇    下一篇

三家国产试剂盒HPV分型检测结果一致性比较分析

余红, 陆其兵   

  1. 224400 江苏省阜宁县人民医院检验科
  • 收稿日期:2018-01-11 出版日期:2019-06-20 发布日期:2019-06-17
  • 作者简介:余红(1971-),女,江苏阜宁人,主任技师,学士,主要从事免疫学及分子生物学研究工作,(E-mail)fnxyuhong@163.com。

A Comparative Analysis of the Agreements of Three Domestic Kits in HPV Typing Tests

YU Hong, LU Qi-bin   

  1. Clinical Laboratory of Funing Hospital,Jiangsu,224400
  • Received:2018-01-11 Online:2019-06-20 Published:2019-06-17

摘要: 目的 比较分析三家国产试剂盒HPV分型检测结果的一致性。方法 收集近2个月来由之江检测的阳性标本82份,阴性标本10份,分别用3家国产试剂进行HPV基因分型检测2次,并进行比较。结果 三家试剂结果重复性分别是之江95.6%,凯普96.7%,安普利95.6%。三家试剂检测结果完全一致率为51.1%(47/92),部分一致率为24.9%(22/92),不一致率为25.0%(23/92)。单一型感染的一致率86.4%(32/37),多重感染完全一致率为22.2%(10/45),部分一致率为48.9%(22/45)。结论 三家国产试剂检测结果一致性不理想。建议患者进行前后治疗的效果对比,最好选在同一家医院进行检测,这样才具有可比性。

关键词: 人乳头瘤病毒, 国产试剂盒, 结果一致性

Abstract: Objective To compare the consistence of HPV typing tests by using three kits that are domestically available. Methods A total of 82 positive samples and 10 negative samples were collected that had been detected with Liferiver kits in recent 2 months and the Results of HPV typing tests were compared for the agreements with the kits purchased from other two companies of Kaipu and Amply. Results The repeatability of the Results in the three domestic kits were 95.6 %,96.7% and 95.6%,respectively. The consistent rate was 51.0%(47/92),the partial consistent rate was 23.9%(22/92),and the inconsistent rate was 25.0%(23/92).The consistent rate of 86.4%(32/37)was seen in detections of single type infection,22.2%(10/45)in multiple infections,and the partial consistent rate found to be 48.9%(22/45). Conclusion of the Results in the three domestic kits varied,and the hospital should be fixed for HPV typing before and after treatments of the infection.

Key words: HPV, Domestic kits, Methodology comparison

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