• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2020, Vol. 22 ›› Issue (1): 6-8.DOI: 10.3969/j.issn.1671-2587.2020.01.002

• 临床输血 • 上一篇    下一篇

原发免疫性血小板减少症患者PBMC中CD200/CD200R1表达及临床意义*

臧艳, 叶辛, 钱宝华   

  1. 200433 上海,第二军医大学长海医院输血科
  • 收稿日期:2018-11-20 出版日期:2020-02-20 发布日期:2020-02-28
  • 通讯作者: 钱宝华,男,主任医师,教授,(E-mail)qianbh1963@163.com。
  • 作者简介:臧艳(1983-),女,江苏丹阳人,副主任技师,硕士,主要从事血液免疫,血液质控研究工作,(Tel)021-31162126。
  • 基金资助:
    *本课题受国家自然科学基金(No.81401358);上海市卫生和计划生育委员会青年基金(No.20144Y0261)资助

Expression of CD200/CD200R1 from Peripheral Blood Mononuclear Cells of Primary Immune Thrombocytopenia and Its Clinical Significance

ZANG Yan, YE Xin, QIAN Bao-hua   

  1. Department of Transfusion Medicine,Changhai Hospital,Second Military Medical University,Shanghai 200433
  • Received:2018-11-20 Online:2020-02-20 Published:2020-02-28

摘要: 目的 检测CD200/CD200R1信号通路分子在原发免疫性血小板减少症(primary immune thrombocytopenia,ITP)患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的表达情况并分析其临床意义。方法 采用实时荧光定量PCR法检测ITP患者组(n=19)和健康对照组(n=19)PBMC中CD200/CD200R1的表达情况。ELISA法检测ITP患者血清中TNF-α,IFN-γ及IL-17细胞因子的表达。分析ITP患者中CD200/CD200R1与细胞因子及血小板计数的相关性。监测有效治疗后ITP患者CD200/CD200R1的变化情况。结果 与健康对照组比,ITP组PBMC中CD200/CD200R1的表达下降,且与患者血小板计数呈正相关。ITP患者血清中细胞因子TNF-α,IFN-γ及IL-17上调,其中TNF-α和IL-17的表达和与CD200及CD200R1的表达量间均呈负相关。经地塞米松治疗有效的ITP患者PBMC中,CD200及CD200R1的表达呈上升趋势。结论 CD200/CD200R1在ITP患者中表达降低,减少了对炎性因子分子表达的抑制作用,因此炎性因子表达增高,从而参与了ITP疾病的发生发展。

关键词: 原发免疫性血小板减少症, CD200/CD200R1, 细胞因子

Abstract: Objective To investigate the expression of CD200/CD200R1 in the PBMC of primary immune thrombocytopenia patients and its clinical significance. Methods The mRNA relative expression of CD200/CD200R1 in ITP patients (n=19) and healthy controls (n=19) were measured by RT-PCR. Then we determined the protein levels of TNF-α, IFN-γ and IL-17 in plasma. We further analyzed the correlation between CD200/CD200R1 expression and platelet counts. The changes of CD200/CD200R1 after effective treatment were also be tested by RT-PCR. Results Compared to healthy controls, CD200/CD200R1 were decreased in ITP patients. TNF-α, IFN-γ and IL-17 protein expressions were increased compared with healthy controls. TNF-α and IFN-γ were negatively correlated with CD200/CD200R1, and the platelet counts in ITP patients were positively correlated with CD200/CD200R1. Moreover, after effective treatment by dexamethasone, the mRNA expression of CD200/CD200R1 showed an obviously increase trend. Conclusion CD200/CD200R1 may be involved in the pathogenesis of ITP and related to the decreased inhibition effect of inflammatory cytokines expression, which provides a new cluefor future immunotherapy.

Key words: Primary immune thrombocytopenia, CD200/CD200R1, Cytokines

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