葡萄糖酸钙和氯化钙制备的血小板凝胶上清对脐带间充质干细胞作用的比较

doi:10.3969/j.issn.1671-2587.2020.01.007

临床输血与检验 ›› 2020, Vol. 22 ›› Issue (1): 28-31.DOI: 10.3969/j.issn.1671-2587.2020.01.007

葡萄糖酸钙和氯化钙制备的血小板凝胶上清对脐带间充质干细胞作用的比较

刘素蕊, 杨晓亚, 高裕华, 王更银

050082 石家庄,联勤保障部队第九八〇医院

收稿日期:2018-07-11 出版日期:2020-02-20 发布日期:2020-02-28
通讯作者: 王更银,主任技师,主要从事血液免疫学、血液临床应用研究,(Tel)0311-87978558。
作者简介:刘素蕊(1982-),女,河北邢台人,主治医师,硕士,主要从事血液制品临床应用和间充质干细胞研究,(Tel)0311-87978562(E-mail)lsr1029@126.com。

PRP Releasate Prepared with Calcium Gluconate and Calcium Chloride in Promotion of Migration and Adhesion of UcMScs

LIU Su-rui, YANG Xiao-ya, GAO Yu-hua, et al

The 980th Hospital of Joint Logistic Support Force,Shijiazhuang 050082

Received:2018-07-11 Online:2020-02-20 Published:2020-02-28

摘要/Abstract

摘要： 目的观察葡萄糖酸钙和CaCl2制备的血小板凝胶上清(platelet-rich plasma releasate,PRP releasate)在脐带间充质干细胞(umbilical cord mesenchymal stromal cells,ucMSCs)迁移和黏附方面的作用。方法应用血细胞分离机收集PRP,10%葡萄糖酸钙或5% CaCl2注射液制备PRP releasate,命名为葡萄糖酸钙-PRP releasate和CaCl2-PRP releasate。以胎牛血清(fetal bovine serum,FBS)培养的细胞作为对照组,采用Transwell板和Matrigel胶分别观察三组细胞的迁移和黏附能力; Western blot检测三组细胞信号分子Cdc42、RhoA和Rac1的表达。结果与FBS培养组相比,两组PRP releasate对细胞迁移和黏附有明显促进作用,葡萄糖酸钙-PRP releasate促迁移最为明显。但两组PRP releasate对细胞黏附的影响无统计学意义。同时,与细胞迁移和黏附相关的Rho家族分子Cdc42、Rac1和RhoA表达上调,葡萄糖酸钙-PRP releasate组最显著。结论较于CaCl2,葡萄糖酸钙-PRP releasate可以更有效促进ucMSCs的迁移。为两者联合应用实现组织修复提供治疗策略。

关键词: 血小板凝胶上清, 脐带间充质干细胞, 迁移, 黏附, GTP酶

Abstract: Objective To observe the role of PRP releasate prepared with calcium gluconate and calcium chloride in promotion of migration and adhesion of human umbilical cord mesenchymal stromal cells (h-ucMSCs). Methods Blood cell separator was used to collect PRP releasate, which was subsequently subjected to the preparation with 10% calcium gluconate or 5% calcium chloride. The h-ucMSCs cultured with FBS was taken as the control. The migration and adhesion of the cells in the three treatments were examined by transwell or matrigel. Western blotting was used to check the expression of Cdc42, RhoA and Rac1. Results The PRP releasate prepared with both calcium gluconate and calcium chloride notably promoted the migration and adhesion of h-ucMSCs compared to the control. Meanwhile, expression of Cdc42, RhoA and Rac1 associated with cell migration and adhesion were elevated. Conclusion PRP releasate prepared with calcium gluconate may improve the migration of h-ucMSCs compared with calcium chloride, suggesting that combined use of both calcium agents with h-ucMSCs might provide a therapeutic strategy for tissue repairs.

Key words: PRP releasate, ucMSCs, Migration, Adhesion, Rho GTPases

中图分类号:

R331.1+4

刘素蕊, 杨晓亚, 高裕华, 王更银. 葡萄糖酸钙和氯化钙制备的血小板凝胶上清对脐带间充质干细胞作用的比较[J]. 临床输血与检验, 2020, 22(1): 28-31.

LIU Su-rui, YANG Xiao-ya, GAO Yu-hua, et al. PRP Releasate Prepared with Calcium Gluconate and Calcium Chloride in Promotion of Migration and Adhesion of UcMScs[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(1): 28-31.

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葡萄糖酸钙和氯化钙制备的血小板凝胶上清对脐带间充质干细胞作用的比较

PRP Releasate Prepared with Calcium Gluconate and Calcium Chloride in Promotion of Migration and Adhesion of UcMScs

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