• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2021, Vol. 23 ›› Issue (3): 340-345.DOI: 10.3969/j.issn.1671-2587.2021.03.012

• 临床检验 • 上一篇    下一篇

23株血液分离产KPC-2肺炎克雷伯菌毒力及同源性研究*

彭成, 王璐, 朱正荣, 赵文紧, 徐元宏   

  1. 237500 皖西卫生职业学院医学技术系(彭成),安徽医科大学附属六安医院检验科(王璐,朱正荣,赵文紧),安徽医科大学第一附属医院检验科(徐元宏)
  • 收稿日期:2021-02-01 出版日期:2021-06-20 发布日期:2021-06-22
  • 通讯作者: 徐元宏,男,主任技师,博士,主要研究方向为病源微生物耐药机制研究,(E-mail)xyhong1964@163.com。
  • 作者简介:彭成(1971-),男,安徽霍山人,副教授,主要从事病原微生物的流行病学及耐药机制研究,(E-mail)276833775@qq.com。共同第一作者:王璐(1985-),女,安徽寿县人,副主任检验技师,硕士,主要从事肠杆菌科细菌耐药机制研究,(E-mail)lawanglu@163.com。
  • 基金资助:
    *本课题受安徽高校自然科学研究重点项目(No.KJ2019A1256)资助

Study on Virulence Feature and Homology of 23 Klebsiella Pneumonia Strains with KPC-2 Gene Isolated from Blood Culture Samples

PENG Cheng, WANG Lu, ZHU Zheng-rong, et al   

  1. Department of Medical Laboratory, Western Anhui Health Vocational College, Lu'an 237005
  • Received:2021-02-01 Online:2021-06-20 Published:2021-06-22

摘要: 目的 了解肺炎克雷伯菌对碳青霉烯类抗菌药物的主要耐药基因携带情况、毒力、分子分型及同源性。方法 收集六安市人民医院2018年1月~2019年12月分离自血培养的非重复CRKP菌株共23株,拉丝试验筛查高毒力菌株;Wzi分型检测荚膜血清型别;聚合酶链式反应检测荚膜多糖合成调节基因(rmpA)、毒力基因及常见碳青霉烯酶基因;多位点序列分型对菌株进行分子分型;脉冲场凝胶电泳技术进行同源性分析。结果 23株菌均携带KPC-2基因,18株拉丝试验阳性且携带rmpA的高毒力菌株。Wzi分型22株为K14.64型,1株为K19型。多位点序列分型为ST11型18株、ST307型4株和ST15型1株,脉冲场凝胶电泳技术进一步将18株ST11型菌株分为3个型别。结论 本研究中肺炎克雷伯菌对碳青霉烯类抗菌药物耐药机制主要是携带KPC-2基因,主要荚膜血清型为K14.64,优势序列型为ST11型,可能存在院内暴发流行。

关键词: 耐碳青霉烯肺炎克雷伯菌, 毒力因子, 多位点序列分型, 同源性

Abstract: Objective To investigate the resistance mechanisms of Carbapenem-resistant Klebsiella pneumonia by genetic analysis, virulence features, phenotypic methods and homology isolated from blood samples. Methods A total of 23 Carbapenem-resistant Klebsiella pneumoniae strains were isolated from blood culture samples between January 2018 and December 2019. Hypervirulent strains and capsular serotypes were identified by string test and Wzi gene sequencing respectively. The exopolysaccharide synthesis regulator gene (rmpA), virulence- associated genes and carbapenemase genes were detected by PCR method. Multilocus sequence typing was used to analyze phenotype of strains. Pulsed field gel electrophoresis was used to analyze the homology of strains. Results All 23 strains of Klebsiella pneumoniae carried KPC-2 genes. There were 18 strains showed positive results of string test and rmpA. Wzi gene sequencing results showed that 22 strains were K14.64 type and 1 strains was K19. All 23 strains were divided into 3 groups by multilocus sequence typing , with the results of ST11 (18/23), ST307 (4/23) and ST15 (1/23). We further identified 18 ST11 strains into three types by pulsed field gel electrophoresis. Conclusions The resistance to carbapenem of Klebsiella pneumoniae strains was mainly associated with KPC-2 gene, which main serotype of capsule was K14.64 and dominant sequence type was ST11 type.

Key words: Carbapenem resistantant Klebsiella pneumoniae, Virulence factors, Multilocus sequence typing Homology

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