• 中国科学论文统计源期刊
  • 中国科技核心期刊
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  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2021, Vol. 23 ›› Issue (3): 371-375.DOI: 10.3969/j.issn.1671-2587.2021.03.017

• 临床研究 • 上一篇    下一篇

上皮性卵巢癌患者血清miR-222-3p、HE4及CA125的表达及其临床意义*

苏红娥, 钟庆吉, 符春丽, 薛万兴, 林元   

  1. 571799 海南西部中心医院妇产科
  • 收稿日期:2020-04-29 出版日期:2021-06-20 发布日期:2021-06-22
  • 作者简介:苏红娥（1978-）,女,海南儋州人,主治医师,本科,主要从事临床妇产科疾病诊治研究,（E-mail）2819641338@qq.com。
  • 基金资助:
    *本课题受海南省医药卫生科研基金项目（No.17A6100-42）资助

Expression and Clinical Significance of MiR-222-3p, HE4 and CA 125 in Serum of Epithelial Ovarian Cancer

SU Hong-e, ZHONG Qing-ji, FU Chun-li, et al   

  1. Department of Obstetrics and Gynecology,Central Hospital of Western Hainan,Hainan 571799
  • Received:2020-04-29 Online:2021-06-20 Published:2021-06-22

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摘要： 目的 探讨上皮性卵巢癌（EOC）患者血清miR-222-3p、血清人附睾蛋白4（HE4）及糖类抗原125（CA125）的表达及其临床意义。方法 选取本院收治的118例EOC患者,105例上皮性卵巢良性肿瘤患者和60例健康女性作为研究对象,采用实时定量PCR法检测miR-222-3p表达水平,化学发光法测定血清HE4及CA125水平。应用受试者工作特征（ROC）曲线分析血清miR-222-3p、HE4及CA125水平对EOC的诊断价值。Pearson相关分析EOC患者血清miR-222-3p表达水平与HE4及CA125的相关性。结果 EOC组血清miR-222-3p（1.62±0.74 vs 0.84±0.35, 0.73±0.24）、HE4（346.20±58.72 pmol/L vs 53.46±11.63 pmol/L, 48.60±9.75 pmol/L）及CA125（307.60±94.25 U/mL vs 26.83±11.56 U/mL , 20.72±9.40 U/mL）水平均明显高于良性组和对照组（均P<0.001）。EOC患者血清miR-222-3p、HE4及CA125表达与临床分期、分化程度及淋巴结转移有关（P<0.01）。ROC曲线显示,血清miR-222-3p、HE4及CA125水平诊断EOC的最佳截断值分别为1.16、187.50 pmol/L、162.80 U/mL,三项联合诊断EOC的曲线下面积（0.948,95%CI：0.887~0.995）最大,其敏感度和特异度为96.8%和89.5%。相关分析显示,EOC患者血清miR-222-3p表达水平与HE4及CA125均呈正相关（r=0.847,P<0.001,r=0.793,P<0.001）。结论 EOC患者血清miR-222-3p、HE4及CA125水平明显升高,三项联合检测对EOC的诊断具有较高的价值。

关键词: 上皮性卵巢癌, miR-222-3p, 血清人附睾蛋白4, 糖类抗原125

Abstract: Objective To investigate the expression of serum miR-222-3p, human epididymal protein 4 (HE4) and carbohydrate antigen 125 (CA125) in epithelial ovarian cancer (EOC) and its clinical significance. Methods A total of 118 EOC patients, 105 in the benign group and 60 in the healthy control group were selected in our hospital. The expression of serum miR-222-3p was detected by real-time quantitative PCR, and the levels of HE4 and CA125 were detected by chemiluminescence. The diagnostic value of serum miR-222-3p, HE4 and CA125 levels to EOC were analyzed by ROC curve. Pearson correlation analysis showed that the expression of miR-222-3p in EOC patients was correlated with HE4 and CA125. Results The levels of serum miR-222-3p (1.62±0.74 vs 0.84±0.35, 0.73±0.24), HE4 (346.20±58.72 pmol/L vs 53.46±11.63 pmol/L, 48.60±9.75 pmol/L) and CA125 (307.60±94.25 U/mL vs 26.83±11.56 U/mL, 20.72± 9.40 U/mL) in EOC group were significantly higher than those in benign group and control group (P<0.001). ROC curve showed that the best cutoff values of serum miR-222-3p, HE4 and CA 125 were 1.16, 187.50 pmol/L and 162.80 U/mL, respectively. The area under the curve (0.948, 95% CI: 0.887~0.995) of the three combined diagnosis of EOC was the largest, and its sensitivity and specificity were 96.8% and 89.5%. Correlation analysis showed that the expression of miR-222-3p in EOC patients were positively correlated with HE4 and CA125（r=0.847,P<0.001,r=0.793,P<0.001）. Conclusion The levels of serum miR222-3p, HE4 and CA125 were significantly increased in EOC patients, and the three combined tests were of high value in the diagnosis of EOC.

Key words: Epithelial ovarian cancer, MiR-222-3p, Human epididymal protein 4, Carbohydrate antigen 125

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