• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2021, Vol. 23 ›› Issue (4): 478-482.DOI: 10.3969/j.issn.1671-2587.2021.04.015

• 临床研究 • 上一篇    下一篇

多发性骨髓瘤循环肿瘤浆细胞的免疫表型特征及其临床意义*

蔡梦洁, 梅仁, 戴兰, 沈文红, 傅琤琤, 朱明清   

  1. 215006 苏州大学附属第一医院,江苏省血液研究所,国家血液系统疾病临床医学研究中心,国家卫生健康委员会血栓与止血重点实验室
  • 收稿日期:2021-04-11 出版日期:2021-08-20 发布日期:2021-08-18
  • 通讯作者: 朱明清,高级实验师,主要从事血液病学研究,（E-mail）zhumingqing@suda.edu.cn。
  • 作者简介:蔡梦洁（1990-）,女,江苏连云港人,技师,硕士,主要从事流式细胞术相关检验工作,（E-mail）1318535809@qq.com。
  • 基金资助:
    *本课题受江苏省科教强卫工程-临床医学中心（No.YXZXA2016002）资助

The Immunophenotype Characteristics and Clinical Significance of Circulating Tumor Plasma Cell （CTPC） in Multiple Myeloma

CAI Meng-jie, MEI Ren, DAI Lan, et al   

  1. Jiangsu Institute of Hematology,National Clinical Research Center for Hematologic Diseases,NHC Key Laboratory of Thrombosis and Hemostasis,The First Affiliated Hospital of Soochow University,Suzhou 215006
  • Received:2021-04-11 Online:2021-08-20 Published:2021-08-18

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摘要： 目的 研究分析多发性骨髓瘤（MM）患者循环肿瘤浆细胞（CTPC）的免疫表型特征及其临床意义。方法 回顾性分析本院75例MM初诊患者的临床资料,采用十色流式细胞术对患者外周血（PB）和骨髓（BM）标本进行免疫表型以及数量相关性检测,将患者分为CTPC阳性组（CTPC+）与CTPC阴性组（CTPC-）。结果 CTPC+组CTPC与BM中单克隆性浆细胞比例呈正相关（γ=0.536 8,P<0.000 1）,但是CTPC+组与CTPC-组BM中单克隆性浆细胞比例无显著差异（P=0.08）;与CTPC-组相比,CTPC+组BM中单克隆性浆细胞CD19表达显著降低（P=0.029）,CD81表达显著升高（P=0.006）,Kappa,Lambda,CD56和CD117表达无统计学差异（P>0.05）;在CTPC+组中,CTPC与BM中单克隆性浆细胞Kappa,Lambda,CD19,CD56,CD81和CD117的表达无统计学差异（P>0.05）。CTPC-组的β2-MG血清水平和遗传学高危组数目显著低于CTPC+组,但是两组CR无显著差异。结论 CTPC能够反映骨髓内肿瘤细胞负荷情况,BM中克隆性浆细胞CD19低表达,CD81高表达与BM外周浸润具有相关性。

关键词: 多发性骨髓瘤, 循环肿瘤浆细胞, 免疫表型, 多参数流式细胞术

Abstract: Objective To explore the immunophenotype characteristics and clinical significance of circulating tumor plasma cell （CTPC） in patients with multiple myeloma（MM）. Methods 75 newly diagnosed MM patients in our hospital were retrospectively analyzed. The immunophenotype and quantitative correlation of peripheral blood （PB） and bone marrow（BM） samples were detected by ten color flow cytometry. The MM patients were divided into CTPC positive group（CTPC+）and CTPC negative group （CTPC-）. Results There was a positive correlation between CTPC and the proportion of mono-clone plasma cells in BM（γ=0.536 8,P<0.000 1）,but there was no significant difference between CTPC+ group and CTPC- group（P=0.08）.Compared with CTPC- group,the expression of CD19 in mono-clone plasma cells in CTPC+ group was significantly decreased（P=0.029）,the expression of CD81 was significantly increased（P=0.006）,and the expression of Kappa,Lambda,CD56 and CD117 was not significantly different（P>0.05）. There was no significant difference between CTPC and BM mono-clone plasma cells in the expression of Kappa,Lambda,CD19,CD56,CD81 and CD117（P>0.05）. The serum level of β2-MG and the number of genetic high risk groups in CTPC-group were significantly lower than those in CTPC+ group,but there was no significant difference in CR between the two groups. Conclusion CTPC could reflect the load of intramedullary tumor cell. The low expression of CD19 and high expression of CD81 in BM clonal plasma cells are correlated with BM infiltration into peripheral system.

Key words: Multiple myeloma, Circulating tumor plasma cell, Immunophenotype, Multi-parameter flow cytometry

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