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临床输血与检验

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (1): 74-79.DOI: 10.3969/j.issn.1671-2587.2022.01.017

• 调查研究 • 上一篇    下一篇

安徽合肥地区单采血小板捐献者血样的HPA基因分型

赵阳, 胡晓玉, 王超, 陈美梅, 赵娜娜, 於娟, 吕蓉   

  1. 230031 合肥,安徽省血液中心
  • 收稿日期:2021-09-15 发布日期:2022-01-27
  • 通讯作者: 吕蓉,女,主任技师,主要从事血液免疫和血液安全研究,（E-mail）lvrong612@126.com。
  • 作者简介:赵阳（1984-）,女,安徽无为人,主管技师,本科,主要从事血液免疫学和输血传染病检测工作,（E-mail）94632801@qq.com。
  • 基金资助:
    *本课题受合肥市科技局2019年自主创新“借转补”项目（No.J2019Y05）资助

HPA Genotyping of Blood Samples from Apheresis Platelet Donors in Hefei,Anhui Province

ZHAO Yang, HU Xiao-yu, LV Rong, et al   

  1. Blood Center of Anhui Province,Hefei 230031
  • Received:2021-09-15 Published:2022-01-27

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摘要： 目的 检测安徽合肥地区单采血小板捐献者血样的HPA基因分型,统计本地区血小板捐献者HPA基因型分布频率。方法 ①采用多重荧光PCR的方法对1 181名18-55周岁的单采血小板捐献者血样进行HPA1-6,10,15,21的基因分型检测。②采用SBT直接测序的方法对22名18-55周岁的单采血小板捐献者血样进行HPA基因型分型检测。结果 发现安徽地区人群中HPA-2,HPA-3,HPA-15最具有多态性,HPA各等位基因型频率分别为：1a∶0.994 1,1b∶0.005 9,2a∶0.946 2,2b∶ 0.053 8,3a∶ 0.574 1,3b∶ 0.425 9,4a∶0.998 7,4b∶0.001 3,5a∶0.981 8,5b∶0.018 2,6a∶0.985 2,6b∶ 0.014 8,10a∶1.0,10b∶0.0,15a∶0.539 4、15b∶0.460 6,21a∶0.992 8、21b∶0.007 2。结论 使用多重荧光方法与SBT直接测序方法HPA基因分型检测结果一致性较好;安徽合肥地区人群的HPA基因分型的多态性与文献中报道的其他地区基本一致,但基因型分布频率有本地区的特点。

关键词: HPA基因分型, 多重荧光PCR法, SBT基因测序, 基因分布频率, 血小板捐献者资料库

Abstract: Objective To detect HPA genotypes of blood samples from apheresis platelet donors in Hefei, Anhui Province,and calculate their distribution frequency in this region. Methods Multiplex PCR was used to characterize HPA1-6,10,15 and 21 genotypes of blood samples from 1181 apheresis platelet donors aged 18-55. Blood samples from 22 apheresis platelet donors aged 18-55 were tested for HPA genotyping using SBT direct sequencing. Results HPA-2, HPA-3 and HPA-15 were the most polymorphic in the Anhui population, and the frequencies of HPA alleles were as follows∶1a∶0.994 1,1b∶0.005 9,2a∶0.946 2,2b∶ 0.053 8,3a∶ 0.574 1,3b∶ 0.425 9,4a∶0.998 7,4b∶0.001 3,5a∶0.981 8,5b∶0.018 2,6a∶0.985 2,6b∶ 0.014 8,10a∶1.0,10b∶0.0,15a∶0.539 4、15b∶0.460 6,21a∶0.992 8、21b∶0.007 2. Conclusion The results of HPA genotyping are similar to those of SBT direct sequencing. The polymorphisms of HPA genotypes in Hefei,Anhui Province basically consist with those in other regions reported in the literature,but the distribution frequency of genotypes is specific to this region.

Key words: HPA genotyping, Multiple fluorescence PCR, SBT gene sequencing, Gene distribution frequency, Platelet donor database

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