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临床输血与检验

临床输血与检验 ›› 2023, Vol. 25 ›› Issue (3): 342-348.DOI: 10.3969/j.issn.1671-2587.2023.03.009

• 临床输血 • 上一篇    下一篇

4例ABO和Rh血型嵌合体的血清学及分子生物学基础研究

许志远, 李晓菲, 刘婷婷, 张烨, 苗天红   

  1. 100088 北京市红十字血液中心
  • 收稿日期:2023-03-13 发布日期:2023-07-10
  • 作者简介:许志远,副主任技师,硕士,主要从事血型血清学和分子免疫血液学研究,（E-mail）zhiyuanxxu@sina.com。

Four Cases of ABO and Rh Blood Group Chimera of Serology and Molecular Biology Basic Research

XU Zhiyuan, LI Xiaofei, LIU Tingting, et al   

  1. Beijing Red Cross Blood Center, Beijing 100088
  • Received:2023-03-13 Published:2023-07-10

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摘要： 目的 研究血型嵌合体的血清学特征分子生物学确认方法。方法 对4例医院输血科送检做ABO和RhD血型的患者,进行ABO和Rh血型鉴定、毛细管离心分离新生红细胞、吸收试验、DTT处理凝集红细胞试验,同时对患者1的血样进行分子生物学研究。结果 患者1：ABO血型为AB/B型嵌合,Rh血型为RhCE基因嵌合;患者2：Rh血型为RhD基因嵌合;患者3：ABO血型为AB/B型嵌合,Rh血型为RhD基因嵌合;患者4：ABO血型为O/B型嵌合,Rh血型为RhCE基因嵌合。结论 在血型血清学上,排除患者有近期输血史,同时又不存在使患者抗原减弱的疾病情况下,ABO和Rh血型出现混合外观现象是其主要特征,实验人员可依据此怀疑患者是有血型嵌合体的可能通过血清学和分子生物学方法进行确认。

关键词: 血型嵌合体, ABO嵌合, Rh嵌合, 毛细管离心, 熔解曲线

Abstract: Objective To study the serological characteristics, the strategy of blood transfusion and the molecular method of chimera. Methods The serology test of ABO and Rh blood group were done to confirm the four patients ABO and RhD blood group from hospital. Separation of newborn red blood cells by capillary centrifugation, absorb test, DTT treatment of agglutination erythrocyte test and molecular biological identification for one case. Results the patient 1, the blood group of ABO is AB/B chimeras, the blood group of Rh is the gene of RhCE chimeras; the patient 2, the blood group of Rh is the gene of RhD chimeras; the patient 3, the blood group of ABO is AB/B chimeras, the blood group of Rh is the gene of RhD chimeras; the patient 4, the blood group of ABO is O/B chimeras, the blood group of Rh is the gene of RhCE chimeras. Conclusions In terms of blood group serology, the main characteristic is mixed appearance on ABO and Rh blood groups in the absence of a recent history of transfusion and a disease that weaken the patient's antigens. Based on this, if there is a suspicious blood group chimera it can be confirmed by serological and molecular biological methods.

Key words: Blood group chimera, ABO chimera, Rh chimera, Capillary centrifugation, Melting curve

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