RhD抗原量子点荧光检测研究

doi:10.3969/j.issn.1671-2587.2022.02.010

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (2): 188-192.DOI: 10.3969/j.issn.1671-2587.2022.02.010

RhD抗原量子点荧光检测研究

杨倩, 陆华, 徐婷

400038 重庆,陆军军医大学第一附属医院输血科（杨倩,徐婷）; 重庆医科大学附属第二医院输血科（陆华）

收稿日期:2021-11-08 发布日期:2022-04-12
通讯作者: 徐婷,女,高级实验师,主要从事临床输血与检验研究,（E-mail）604537938@qq.com。共同通信作者：陆华,男,副主任医师,副教授,主要从事临床输血与检验研究,（E-mail）1580509532@qq.com。
作者简介:杨倩（1993-）,女,重庆人,检验技师,本科,主要从事临床输血与检验研究,（E-mail）771052231@qq.com。

Develop a Quantum Dots Fluorescent Detection of RhD Blood Group

YANG Qian, LU Hua, XU Ting

Department of Transfusion, Southwest Hospital, Army Medical University, Chongqing 400038

Received:2021-11-08 Published:2022-04-12

摘要/Abstract

摘要： 目的建立一种新型的RhD抗原检测方法。方法采用量子点-抗体复合物和C1q-磁珠分离RhD抗原,根据反应前后的荧光强度比值（Ratio value,R值）对RhD抗原进行检测;对160例临床标本的RhD血型分型,确定量子点荧光检测法的检测性能。结果当红细胞浓度处于1×10¹⁰~1×10¹¹/L,35 min内可完成RhD血型抗原的检测;检测体系的组内和组间变异系数分别为3.86%和5.06%。通过量子点荧光检测法鉴定160例临床标本的RhD血型,与试管法相比符合率为100%;ROC曲线分析显示,当Cut-off值为1.610时,量子点荧光检测法在临床样本中具有较高的敏感性和特异性。结论量子点荧光RhD血型检测可作为常规RhD抗原检测方法的一种有效补充。

关键词: Rh血型系统, D抗原, 量子点, 荧光检测

Abstract: Objective To develop a new method for RhD blood group typing. Methods In the quantum dots fluorescence detection, blood group D antigen was seperated by quantum dots-antibody complexes and magnetic beads, and identified according to the ratio of fluorescence intensity before and after the reaction （Ratio value）. The detection performance of this method was validated by blood typing of 160 clinical specimens. Results When the RBC concentration is at 1×10¹⁰~1×10¹¹/L the detection of RhD blood group antigen could be completed within 35 minutes. The intra- and inter-group variation coefficients of the detection system were 3.86% and 5.06%, respectively. Compared with the tube test method, the accuracy of the quantum dots fluorescence detection was 100% by the RhD blood group results of 160 clinical samples. ROC curve analysis showed that when the Cut-off value was 1.610, the quantum dot fluorescence detection method had the highest sensitivity and specificity. Conclusion Quantum dot fluorescent RhD blood group detection can be used as an effective supplement to conventional RhD antigen detection methods.

Key words: Rh blood group system, D antigen, Quantum dots, Fluorescence detection

中图分类号:

R446.6
R331

杨倩, 陆华, 徐婷. RhD抗原量子点荧光检测研究[J]. 临床输血与检验, 2022, 24(2): 188-192.

YANG Qian, LU Hua, XU Ting. Develop a Quantum Dots Fluorescent Detection of RhD Blood Group[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(2): 188-192.

参考文献

[1] MARKS L.Monoclonal antibodies and the transform ation of blood typing[J]. mAbs,2014,6(6):1362-1367.
[2] FIGUERAS-ALOY J,RODRÍGUEZ-MIGUÉLEZ J M,IRIONDO-SANZ M,et al.Intravenous immunoglobulin and necrotizing enterocolitis in newborns with hemolytic disease[J]. Pediatrics,2010,125(1):139-144.
[3] ZUPPA A A,CARDIELLO V,LAI M,et al.ABO hemolytic disease of the fetus and newborn:an iatrogenic complication of heterologous assisted reproductive technology-induced pregnancy[J]. Transfusion,2010,50(10):2102-2104.
[4] ALI M A,SRIVASTAVA S,PANDEY M K,et al.Protein-conjugated quantum dots interface:binding kinetics and label-free lipid detection[J]. Anal Chem,2014,86(3):1710-1718.
[5] TENÓRIO D P L A,ANDRADE C G,CABRAL FILHO P E,et al. CdTe quantum dots conjugated to concanavalin A as potential fluorescent molecular probes for saccharides detection in Candida albicans[J]. J Photochem Photobiol B,2015,142:237-243.
[6] BILAN R,NABIEV I,SUKHANOVA A.Quantum dot-based nanotools for bioimaging,diagnostics,and drug delivery[J]. Chembiochem,2016,17(22):2103-2114.
[7] ZHANG P F,BAO Y,DRAZ M S,et al.Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay[J]. Int J Nanomedicine,2015,10:6161-6173.
[8] LIU L F,WU S M,JING F X,et al.Bead-based microarray immunoassay for lung cancer biomarkers using quantum dots as labels[J].Biosens Bioelectron,2016,80:300-306.
[9] 陈宏础, 叶应妩,王毓三, 主编.全国临床检验操作规程(第2版)[M], 南京: 东南大学出版社, 1997:93-95.
[10] 桂嵘, 张志昇, 王勇军, 主编. 输血相容性检测及疑难病例分析[M]. 北京: 人民卫生出版社, 2018: 77-83.
[11] YALCIN S E,LABASTIDE J A,SOWLE D L,et al.Spectral properties of multiply charged semiconductor quantum dots[J]. Nano Lett,2011,11(10):4425-4430.
[12] PATNAIK S K,HELMBERG W,BLUMENFELD O O.BGMUT database of allelic variants of genes encoding human blood group antigens[J].Transfus Med Hemother,2014,41(5):346-351.
[13] BRANCH D R.Anti-A and anti-B:what are they and where do they come from?[J]. Transfusion,2015,55(Suppl 2):S74-S79.
[14] XU T,ZHANG Q,FAN Y H,et al.Quantitative and multiplexed detection for blood typing based on quantum dot-magnetic bead assay[J].Int J Nanomedicine,2017,12:3347-3356.
[15] PRAGER M.Molecular genetic blood group typing by the use of PCR-SSP technique[J]. Transfusion,2007,47(1 Suppl):54S-59S.
[16] FICHOU Y,AUDRÉZET M P,GUÉGUEN P,et al. Next-generation sequencing is a credible strategy for blood group genotyping[J]. Br J Haematol,2014,167(4):554-562.
[17] HOPP K,WEBER K,BELLISSIMO D,et al.High-throughput red blood cell antigen genotyping using a nanofluidic real-time polymerase chain reaction platform[J]. Transfusion,2010,50(1):40-46.

RhD抗原量子点荧光检测研究

Develop a Quantum Dots Fluorescent Detection of RhD Blood Group

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

关于本刊

作者中心

在线期刊

访问统计

联系我们

[1]	李明浩, 杨佳璇, 李艾静, 朱自严, 叶璐夷. 1例部分D表型献血者RhD抗原表位及分子机制研究*[J]. 临床输血与检验, 2022, 24(1): 16-21.
[2]	张保萍, 张嵘, 王文婷, 安群星, 尹文, 胡兴斌. 谱细胞鉴定剂量效应分析偏差致抗-C抗体漏检1例[J]. 临床输血与检验, 2021, 23(2): 236-239.
[3]	范亮峰, 陆琼, 姜跃琴, 王中英, 向东. 人血液经56℃孵育处理后溶血率及血型抗原抗体变化^*[J]. 临床输血与检验, 2021, 23(1): 54-60.
[4]	吴凡, 庄乃保, 梁爽, 梁延连, 苏宇清. RhD多肽抑制抗原抗体结合的效果*[J]. 临床输血与检验, 2020, 22(1): 23-27.
[5]	张秋会, 胡兴斌, 安群星, 张婧, 尹文. Rh联合Duffy血型系统的抗体鉴定及输血治疗1例[J]. 临床输血与检验, 2017, 19(1): 93-94.
[6]	党鑫堂,吴筱莹,王学东,伍昌林,朱奕,李妙珊,邵超鹏. 1例部分D表型孕妇抗-D及基因型分析^*[J]. 临床输血与检验, 2016, 18(6): 521-524.
[7]	朱自严. RhD抗原的鉴定我们该做的多深入[J]. 临床输血与检验, 2016, 18(4): 305-311.