流式方法检测患者红细胞上IgA、IgG类抗体的临床意义

doi:10.3969/j.issn.1671-2587.2025.01.007

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (1): 54-58.DOI: 10.3969/j.issn.1671-2587.2025.01.007

流式方法检测患者红细胞上IgA、IgG类抗体的临床意义

刘曦¹, 沈雨青², 孙瑛¹, 张玉宇¹, 朱心怡¹, 向东¹

¹上海市血液中心,上海 200051;
²厦门大学附属妇女儿童医院,福建厦门 361000

收稿日期:2024-11-01 发布日期:2025-02-25
通讯作者: 向东,主要从事免疫血液学研究,（E-mail）xiangdong@sbc.org.cn。
作者简介:刘曦,主要从事免疫血液学研究,（E-mail） liuxi@sbc.org.cn。

Detection of IgG, IgA Antibodies on Red Blood Cells by Flow Cytometry

LIU Xi¹, SHEN Yuqing², SUN Ying¹, ZHANG Yuyu¹, ZHU Xinyi¹, XIANG Dong¹

¹Shanghai Blood Center, Shanghai 200051;
²Women and Children’s Hospital, School of Medicine, Xiamen University, FuJian Xiamen 361003

Received:2024-11-01 Published:2025-02-25

摘要/Abstract

摘要： 目的目前免疫血液学检测中普遍缺乏针对抗IgA的检测,可能存在IgA类血型抗体的漏检。因此需要了解患者人群红细胞上IgA、IgG抗体的分布,分析红细胞上存在IgA抗体对患者贫血可能造成的影响。方法 2023年3月—10月接收的申请配血的患者样本,采用血清学方法完成常规血清学检测之后,运用流式细胞技术检测IgA和IgG抗体。结果在71例可以获得完整的病史资料病例中,通过流式方法检出56例IgA抗体,占比明显高于以往国内、外文献报道;检出56例IgG抗体,流式方法检测抗体的阳性率高于常规血清学方法。结论有临床表现但实验室检测直抗试验阴性的疑似自身免疫性溶血性贫血或溶血性输血反应病例,在日常检测工作中增加IgA抗体检测,可以减少漏检。

关键词: IgA抗体, 流式细胞术, 免疫球蛋白, 自身免疫性溶血性贫血, 溶血性输血反应

Abstract: Objective In immunohematological tests, IgA blood type antibodies may be missed due to the general lack of anti-IgA. It is necessary to understand the distribution of IgA and IgG antibodies on red blood cells of patients to analyze the potential influence of IgA antibodies on anemia in patients. Methods The Samples collected from patients applying for crossmatching between March and August 2023 were tested for IgA and IgG antibodies using flow cytometry after routine serological testing. Results In 71 cases, 56 IgA antibodies were detected by flow cytometry, which was significantly higher than that reported in domestic and foreign literatures; 56 IgG antibodies were detected. The positive rate was higher than that of conventional serological methods. Conclusion In cases of suspected autoimmune hemolytic anemia or hemolytic blood transfusion reaction with clinical manifestations but negative direct antibody tests, IgA antibody testing should be added to reduce missed detection.

Key words: IgA antibody, Flow cytometry, Immunoglobulin, Autoimmune hemolytic anemia, Hemolytic transfusion reaction

中图分类号:

R446

刘曦, 沈雨青, 孙瑛, 张玉宇, 朱心怡, 向东. 流式方法检测患者红细胞上IgA、IgG类抗体的临床意义[J]. 临床输血与检验, 2025, 27(1): 54-58.

LIU Xi, SHEN Yuqing, SUN Ying, ZHANG Yuyu, ZHU Xinyi, XIANG Dong. Detection of IgG, IgA Antibodies on Red Blood Cells by Flow Cytometry[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(1): 54-58.

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流式方法检测患者红细胞上IgA、IgG类抗体的临床意义

Detection of IgG, IgA Antibodies on Red Blood Cells by Flow Cytometry

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