• 中国科学论文统计源期刊
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临床输血与检验 ›› 2025, Vol. 27 ›› Issue (4): 552-556.DOI: 10.3969/j.issn.1671-2587.2025.04.018

• 个例报告 • 上一篇    下一篇

抗-Dia和抗-Wra混合抗体检出报告一例

赵小琳, 张成鑫, 王秋实   

  1. 中国医科大学附属盛京医院,辽宁沈阳 110004
  • 收稿日期:2025-03-11 发布日期:2025-08-22
  • 通讯作者: 王秋实,主要从事临床输血,大量用血管理,疑难血型鉴定等方面研究,(E-mail)qswang@cmu.edu.cn。
  • 作者简介:赵小琳,主要从事血型血清学技术研究,(E-mail)zhaoxiaolin0120@163.com。

A Case of Mixed Antibodies Against both Anti-Dia and Anti-Wra

ZHAO Xiaolin, ZHANG Chengxin, WANG Qiushi   

  1. Shengjing Hospital Affiliated to China Medical University, Liaoning Shenyang 110004
  • Received:2025-03-11 Published:2025-08-22

摘要: 目的 对一例6个月前有输血史,体内存在意外抗体的患者进行意外抗体鉴定。方法 应用2组筛选细胞及2组谱细胞对患者意外抗体筛查和鉴定,利用毛细管超高速离心分离新老细胞技术确定直接抗人球蛋白试验(DAT)阳性类型,对酸放散液再次进行抗体鉴定,结合患者抗原分型确定抗体特异性。结果 患者游离血浆中检出抗-Dia和抗-Wra抗体,远心端DAT分型为抗-IgG1阳性,抗-C3d阴性,近心端DAT分型为抗-IgG阴性,抗-C3d阴性,放散液检出抗-Dia和抗-Wra抗体。结论 患者体内存在抗-Dia和抗-Wra抗体,放散液也检出抗-Dia和抗-Wra抗体,该患者产生了同种抗-Dia和抗-Wra抗体。

关键词: Diego血型系统, 抗-Dia, 抗-Wra, 意外抗体

Abstract: Objective To identify irregular antibodies in a patient with a history of blood transfusion six months prior and the presence of DAT. Methods Two sets of antibody screening cells and two sets of screening panel cells were utilized to screen and identify irregular antibodies. Capillary ultra-high-speed centrifugation was employed to separate new and old cells, thereby determining the positive type of direct anti-human globulin. Antibody identification was subsequently performed in an acid release solution, and antibody specificity was determined in conjunction with patient antigen typing. Results Anti-Dia and anti-Wra antibodies were detected in the patient. Distal DAT typing was positive for anti-IgG1 and negative for anti-C3d. Proximal DAT typing was negative for both anti-IgG and anti-C3D. Anti Dia and anti-Wra antibodies were detected in the release fluid. Conclusion The patient exhibited the presence of anti-Dia and anti-Wra antibodies. These antibodies were confirmed in the release fluid, indicating that the patient produced identical anti-Dia and anti-Wra alloantibodies.

Key words: Diego Blood group system, Anti-Dia, Anti-Wra, Alloantibody

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