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临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (1): 55-58.DOI: 10.3969/j.issn.1671-2587.2017.01.016

• 临床输血 • 上一篇    下一篇

邢台地区血小板志愿捐献者HPA基因资料库的建立

吕冉, 刘郁, 王朋蕊, 高秀俊, 王志森   

  1. 054000 河北省邢台市中心血站（吕冉,刘郁,高秀俊,王志森）; 隆尧县医院（王朋蕊）
  • 收稿日期:2016-08-28 出版日期:2017-02-20 发布日期:2017-03-02
  • 作者简介:吕冉（1982-）,女,河北邢台人,主管检验师,学士,主要从事免疫血液学工作,（Tel）15033199688,0319-2158864（E-mail）xuelv1980@163.com。

Establishment of HPA Gene Database for Platelet Donors in Xingtai Area of Hebei

LV Ran, LIU Yu, WANG Peng-rui, et al. Xingtai   

  1. Central Blood Station of Hebei Province,Xingtai,054000
  • Received:2016-08-28 Online:2017-02-20 Published:2017-03-02

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摘要： 目的 对邢台地区血小板志愿捐献者进行人类血小板抗原（human platelet antigen,HPA）基因分型,建立已知HPA基因型的血小板捐献者资料库。方法 采用聚合酶链反应-序列特异性引物（polymerase chain reaction-sequence specific primers,PCR-SSP）方法对随机抽取的150例血小板捐献者进行HPA-1~6和HPA-15抗原系统共14个抗原基因分型。结果 邢台地区血小板志愿捐献者HPA的基因频率分别为：HPA-1a 0.953,1b 0.010;2a 0.953,2b 0.086;3a 0.596,3b 0.394;4a 1.000,4b 0;5a 0.967,5b 0.015;6a 0.986,6b 0.010;15a 0.475,15b 0.506。结论 邢台地区血小板志愿捐献者HPA-1~6和HPA-15抗原系统的基因频率符合Hardy-weinberg遗传定律,与其他地区和国家的同类资料相比显示出种族和地域性差异,应建立邢台地区已知HPA基因型的血小板捐献者资料库。

关键词: 邢台地区, HPA基因型, 血小板血型

Abstract: Objective The HPA genotyping of platelet donors in xingtai area was performed,and the HPA genotype of the platelet donors was established. Method PCR-SSP Method was used to randomly select 150 platelet donors who carry out a total of 14 antigens of HPA-1-6 and HPA-15 . Result The gene frequencies were found to be 0.953 and 0.010 for HPA-1a and -1b：0.951 and 0.086 for HPA-2a and -2b：0.596 and 0.394 for HPA-3a and -3b：1.000 and 0.000 for HPA-4a and -4b：0.967 and 0.015 for HPA-5a and -5b：0.986 and 0.010 for HPA-6a and -6b：0.475 and 0.506 for HPA-15a and -15b. Conclusion The gene frequencies of HPA-1-6 and HPA-15 antigens in xingtai area of Hebei province coincide with the Hardy-Weinberg genetic law,which shows difference among nationalities and regions.This is the first establishment of HPA genotype database in xingtai.

Key words: Xingtai region, HPA genotyping, Blood platelet

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