• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (4): 316-319.DOI: 10.3969/j.issn.1671-2587.2016.04.004

• 临床输血研究 • 上一篇    下一篇

不同保存期的去白细胞悬浮红细胞上清液中微粒粒径分布研究

  

  1. 528000  广东省佛山市中心血站(邓智豪,官亚妹,伍伟健);暨南大学生物医学工程系(陀伟为,黄耀熊)
  • 出版日期:2016-08-20 发布日期:2016-08-26
  • 基金资助:
    本课题受广东省佛山市卫计局科研课题项目(No.2014162)资助

The Particle Size Distribution in Leukocyte-reduced Red Blood Cell Suspensions in Different Durations of  Sample Storage 

  1.  Foshan Central Blood Station, Foshan 528000
  • Online:2016-08-20 Published:2016-08-26

摘要:

 目的  探索库存去白细胞悬浮红细胞(去白悬浮红)随着保存时间的延长,红细胞破损或溶血而产生的细胞碎片以及微粒变化,为输血安全提供启示性实验基础。方法  取不同保存天数(3、7、14和21 d)的库存去白悬浮红进行上清液制备,应用动态光散射仪对样品中的各种微粒进行粒径分布分析。 结果  3 d和7 d的库存去白悬浮红上清液样品中的微粒主要为10~25 nm微粒;从14 d开始,上清液样品中出现了更多 150~300 nm 的微粒,到 21 d时,150~300 nm 范围内的微粒占绝大部分。结论  可通过测定库存红细胞制品上清液中微粒的粒径变化来评估红细胞的储存损伤程度,进一步探讨新的溶血评价方法。

关键词: 去白悬浮红细胞, 动态光散射, 微粒, 溶血评价

Abstract:  Objective  As storage time  extension, fragments and particles changes need to be explored for breakage and hemolysis of aleukocytic red blood cell suspensions, so as to provide a  experimental basis  for the transfusion safety. Methods  Aleukocytic suspending red blood cells from stocks were prepared in different storage  days (3d, 7d, 14d and 21d).  Particles in the samples according to the grain size distribution were analyzed through dynamic light scattering. Results Particles with the size  from 10 to25 nm in the supernatant of  the leukocyte-free red blood cells on 3d and 7d, while particles of 150~300 nm in size were predominantly noted after 3 weeks storage. Conclusion  Particle size measurements in the RBC suspension of leukocyte reduced storage samples would be useful for evaluation the hemolysis in RBC transfusion.

Key words:  Aleukocytic suspending red blood cell ;Quasi-elastic Laser Scattering;   , Particle;   , Hemolysis evaluation