• 中国科学论文统计源期刊
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临床输血与检验

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (4): 312-315.DOI: 10.3969/j.issn.1671-2587.2016.04.003

• 临床输血研究 • 上一篇    下一篇

洗涤红细胞中K+变化的影响因素研究

李蓓, 李继明, 范丽莉   

  1. 266071   山东省青岛市中心血站
  • 出版日期:2016-08-20 发布日期:2016-08-26
  • 基金资助:
    本课题受山东省医药卫生科技发展计划项目（No.2014WS0221）资助

Study on the Influencing Factors of K+ Concentration in the Washed Red Blood Cells

  1. Qingdao Blood Center, Qingdao 266071
  • Online:2016-08-20 Published:2016-08-26

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摘要：  目的  研究用MAP保养液保存的去白悬浮红细胞制备洗涤红细胞，其保存期内K+浓度及其他相关性指标的变化趋势，为临床合理输血提供依据。方法  选取去白和不去白悬浮红细胞制备MAP洗涤红细胞，检测其在不同保存期的K+和Na+浓度、游离血红蛋白浓度、红细胞渗透脆性、白细胞去除率、红细胞回收率等指标。结果  去白悬浮红细胞较未去白悬浮红细胞制备的洗涤红细胞K+浓度升高速度慢，洗涤红细胞在保存期内，Na+浓度逐渐下降，游离血红蛋白浓度逐渐升高，红细胞渗透脆性逐渐加强。结论  临床输血时根据患者的不同情况有针对性选择不同保质期的洗涤红细胞，特别是针对高钾血症和新生儿患者等可优先选择即时洗涤红细胞（24 h内），而对于预防血浆过敏及白细胞副反应等患者，可选用MAP保存洗涤红细胞。

关键词: 洗涤红细胞, 钾离子, 相关性研究

Abstract: Objective  To study the preparation of washed red blood cells(RBCs) with MAP maintenance solution, and to provide a guidance for clinical rational blood transfusion. Methods  MAP washed  RBCs were prepared from the  leukocyte reduced/containing   RBCs suspension  K+ and Na+ concentration, free hemoglobin, erythrocyte osmotic fragility, leukocyte removal rate, erythrocyte recovery rate and other indicators were detected. Results  The K+ concentration of washed  RBCs was lower than that of  RBCs. The Na+ concentration decreased whereas the free hemoglobin concentration increased gradually. The RBCs osmotic fragility was increased, indicating that the increase of K+ concentration may be related to the imbalance of sodium potassium pump, hemolysis and osmotic fragility of erythrocytes. Conclusion  According to the different conditions of patients, especially of the patients with high serum potassium and newborn infants, the freshly washed red blood cells are recommended for use to prevent the plasma allergy and the side reaction of MAP cells.

Key words: Washed red blood cell , ；K+ , ； Correlation study