关键词: 添加液, 去白洗涤红细胞, 质量

Abstract: Objective To investigate the processing feasibility and quality after the storage of the leukocytes removed red blood cells in additive solution. Methods One bag of ,leukocyte removed red blood cells in additive solution was divided into six equal volumes. One of them was stored in refrigerator at 4±2℃, without washing as control group whereas the others were washed by saline or MAP, suspended in saline, MAP or ACD. Saline washed-saline suspended group, saline washed-MAP suspended group, saline washed -ACD suspended group, MAP washed -MAP suspended group, and MAP washed -ACD suspended group were taken for experiments. Samples were subjected to different preservation time （0~35 d） after preparation. The variance analysis was conducted according to the factorial design of repeated measurement of different groups and time intervals. Results HGB content, supernatant protein content, residue leukocytes and sterility test were all in line with the national standards. The K⁺ concentration, pH value and FHb content were statistically different at each “time”and “group”. Conclusion The leukocyte-removed erythrocytes can be washed and suspended in added liquid for a long time with viability. The suitable washed RBCs products are recommended in clinical transfusion. The washed red cells suspended in saline within 24 hours or in additive solution stored within three days would be appropriate.

Key words: Additive solution, Washed red blood cells leukocytes reduced, Quality