乙脑病毒E蛋白抗体在THP-1细胞中对增强DENV-Ⅱ感染的作用研究*

doi:10.3969/j.issn.1671-2587.2020.06.009

临床输血与检验 ›› 2020, Vol. 22 ›› Issue (6): 594-598.DOI: 10.3969/j.issn.1671-2587.2020.06.009

乙脑病毒E蛋白抗体在THP-1细胞中对增强DENV-Ⅱ感染的作用研究^*

徐倩, 姜黎明, 欧阳春

430033 湖北省第三人民医院(徐倩,欧阳春); 中国医学科学院＆北京协和医学院医学生物学研究所(姜黎明)

收稿日期:2020-06-15 出版日期:2020-12-20 发布日期:2021-01-18
通讯作者: 欧阳春,男,主任技师,学士,主要从事临床输血检验与血液管理研究,(E-mail)3172753103@qq.com。
作者简介:徐倩(1983-),女,湖北武汉人,主管技师,硕士,主要从事临床输血检验与血液管理研究,(Tel)15926407367。
基金资助:
*本课题受国家“十二五”“重大新药创新”科技重大专项(No.2018ZX09114-402)资助

Study on the Enhancement of DENV-Ⅱin THP-1 Cells by Japanese Encephalitis Virus E Protein Antibody

XU Qian, JIANG Li-ming, OUYANG Chun

The Third People's Hospital of Hubei Province,Wuhan 430033

Received:2020-06-15 Online:2020-12-20 Published:2021-01-18

摘要/Abstract

摘要： 目的利用THP-1细胞研究梯度稀释的乙脑病毒(Japanese encephalitis virus,JEV)E蛋白抗体对Ⅱ型登革病毒(Dengue virus,DENV-Ⅱ)复制能力的影响。方法采用不同稀释度的JEV-E抗体与分离自云南西双版纳重症患者血清中的DENV-Ⅱ复合感染人THP-1细胞,构建DENV-Ⅱ病毒荧光定量PCR检测标准曲线,利用实时荧光定量PCR检测不同稀释度的JEV-E抗体与DENV-Ⅱ以不同MOI (Multiplicity of infection) 复合感染人THP-1细胞的病毒拷贝数。结果实时荧光定量PCR检测发现1/4稀释度浓度的JEV-E抗体促进DENV-Ⅱ在人THP-1细胞内复制,而高浓度和低浓度JEV-E抗体对DENV-Ⅱ在人THP-1细胞内的复制无明显促进作用。结论同为黄病毒属的JEV病毒E蛋白抗体能够诱发DENV-Ⅱ在人THP-1细胞产生抗体依赖增强感染现象,为后续研究黄病毒间的抗体依赖增强感染现象奠定了理论基础。

关键词: 登革病毒, 乙脑病毒, 抗体依赖增强感染, THP-1

Abstract: Objective To study the effct of gradient dilution of Japanese encephalitis virus (JEV) E protein antibody on the replication ability of dengue virus type Ⅱ(DENV-Ⅱ) in THP-1 cells.Methods Human THP-1 cells were co-infected with JEV-E antibodies of different dilution and DENV-Ⅱisolated from the serum of critically ill patients in Xishuangbanna,Yunnan Province.The standard curve of fluorescent quantitative PCR detection of DENV-Ⅱvirus was constructed.We used different dilution of JEV-E antibodies and DENV-Ⅱat different MOI (Multiplicity of infection) to infect THP-1 cells,then DENV-Ⅱvirus copy number was detected by real-time fluorescent quantitative PCR.Results We found 1/4 dilution degrees of JEV-E antibody concentration could promote DENV-Ⅱreplication in THP-1 cells,while high and low concentration of JEV-E antibody had no significant effect on DENV-Ⅱvirus replication in THP-1.Conclusion Flaviviruses JEV E protein antibody can induce antibody dependent enhanced infection of DENV-Ⅱin human THP-1 cells,which lays a theoretical foundation for the further study of antibody dependent enhanced infection among flaviviruses.

Key words: Dengue virus, Japanese Encephalitis virus, Antibody-dependent enhanced infection, THP-1

中图分类号:

R373.3⁺3

徐倩, 姜黎明, 欧阳春. 乙脑病毒E蛋白抗体在THP-1细胞中对增强DENV-Ⅱ感染的作用研究^*[J]. 临床输血与检验, 2020, 22(6): 594-598.

XU Qian, JIANG Li-ming, OUYANG Chun. Study on the Enhancement of DENV-Ⅱin THP-1 Cells by Japanese Encephalitis Virus E Protein Antibody[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(6): 594-598.

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乙脑病毒E蛋白抗体在THP-1细胞中对增强DENV-Ⅱ感染的作用研究^*

Study on the Enhancement of DENV-Ⅱin THP-1 Cells by Japanese Encephalitis Virus E Protein Antibody

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