Abstract: ObjectiveTo investigate the genotypes of HCV among blood donors in Fuzhou city of China. MethodsAntibodies against HCV and RNA were detected by ELISA and nucleic acid technology(NAT), and the possitive samples were amplified by PCR. The PCR-positive samples were subjected to 5′ UTR gene DNA sequencing, and HCV genotypes were determined by phylogenetic analysis. Results92 samples were both reactive by ELISA and NAT screening, and 52 of them were amplified successfully by PCR, all of these samples were determined the genotypes by sequencing 5’ UTR gene, and the results were 6a [(19/52)36.54%], 1b [(18/52)34.62%], 3a [(6/52) 11 .54%], 1a [(2/52)3.85%], 3b [(2/52)3.85%], others [(5/52)9.60%]. Conclusions6a and 1b were predominant HCV genotypes in the blood donors in Fuzhou city of China.

Key words: HCV, Phylogenetic, analysis, Genotype