Abstract: ObjectiveTo explore the effects on biological activity of coagulation factor in cryoprecipitate by using dry plasma and waterbath plasma melting instrument to melt cryoprecipitate and the length of melting time. Guiding transfusion department to protect the activity of coagulation factor better in cryoprecipitate and choose the right melting instrument as well as the opportune time. Providing a theoretical basis to achieve the best effect of melt. MethodChoosing 140 bags cryoprecipitate (specifications 1.0 U / bag) provided by Kunming Blood Center of Yunnan and the acquisition time is similar (not more than five days). Choosing 80 bags cryoprecipitate as test group 1 and using water-soluble plasma melting instrument to melt. Choosing the remaining 60 bags cryoprecipitate as test group 2 and using dry plasma melting instrument to melt. Using CA-1500 automatic coagulation analyzer to test FⅧ, VWF antigen, PT, TT, APTT and Fib of 140 copies cryoprecipitate samples of which are melted 10 minutes, 20 minutes, 30 minutes in different plasma melting instruments of 37 ℃. ResultThe effects of dry and water bath plasma melting instruments and various detection index compared with no significant difference, P> 0.05. But with the extension of melting time will lead to the APTT, FⅧ, VWF antigen activity decreased, P<0.05, the difference is significant. ConclusionProlonged the time of melting plasma will cause a decrease in coagulation factor FⅧ , VWF antigen activity and the effectiveness of APTT prolonged. There is a significant difference. In order to ensure the clinical blood transfusion is safe and effective, we recommend the best melting time of cryoprecipitate as 10 minutes.

Key words: Different, melting, method, Cold, precipitation, Clotting, factor, Melting, time