Abstract: Objective To study the effect on the quality of cryoprecipitation from fresh frozen plasma which was centrifugated with high speed once or twice. We detected the content of free hemoglobin, factor Ⅷ and fibrinogen in cryoprecipitate. Methods 40 units whole blood（200 mL per-unit）were divided into two groups. The fresh frozen plasma in control group was centrifugated for once, and that in experimental group was centrifugated twice. Then the following separations were in accordance with national standards. We got the cryoprecipitate of two groups 14 days later, then detected the content of free hemoglobin, factor Ⅷ and fibrinogen in cryoprecipitate. Results Free hemoglobin in the experimental group (17.64±5.60) mg/L is lower than that in the control group (25.75±9.78) mg/L, (P<0.05); and in the experimental group, the content of factor Ⅷ is (53.26±6.08) IU/unit, which is higher than that in the control group （47.34±9.14）IU/ unit（P<0.05）; and the content of fibrinogen is（162±29.01）mg/ unit, which is higher than that in the control group (147.28±43.39) mg/ unit, （P>0.05）. Conclusion By additional high speed centrifugation, more residual red blood cells can be removed from fresh frozen plasma, which may improve the quality of the cryoprecipitation, and increase the content of factor Ⅷ and fibrinogen.

Key words: Fresh frozen plasma, Cryoprecipitate, Free hemoglobin, Factor &#x02167, Fibrinogen