Abstract: Objective To investigate the feasibility of the cryoprecipitation using the dewhite plasma within 24h . Method A total of 180 bags of blood （400mL/bag） were collected and stored under 22℃ from May 2016 to February 2017. The blood was divided into six groups （A1,A2,B1,B2,C1,C2）,with 30 bags in each. The concentrated platelets were prepared by albuginea method within 8 h after collection in group A1 and within 20 h-24 h in groups A2,B1,B2,C1 and C2,respectively. The upper-albuginea plasma was separated and quickly frozen as the source for cryoprecipitation. The contents of factor VIII （FVIII） and Fibrinogen （FIB） in cryoprecipitation were detected by coagulation analyzer,and the influence of different preparation time （groups A1 and A2）,quick-freezing methods （groups B1 and B2） and cryoprecipitation preparation methods （groups C1 and C2） on the contents of FVIII and FIB in cryoprecipitation and the qualified rates were compared. ResultThe influence of two storage times on the quality of FVIII and FIB in cryoprecipitation was not statistically significant （P>0.05）. The freezing by instant freezers and preparation methods of cryoprecipitation gave rise to a remarkable impact on the quality of FVIII（P<0.05）. The FIB in groups A1,A2,B1,B2,C1 and C2 was qualified but a statistical difference （P<0.05） was noted FIB between groups C1 and C2. Conclusion The dewhite plasma within 24 h at 22℃ after concentration of platelets might be used as the source for cryoprecipitation,the temperature of the plasma and preparation method of cryoprecipitation play a crucial role in guaranteeing the quality of FⅧ and FIBs.

Key words: Buffy- coat -removed plasma, Cryoprecipitation, VIII factor, Fibrinogen, Quality control