Abstract: Objective To explore parameter settings of ELISA equipment which is commonly used by blood stations on STAR automatic pipetting instrument,in order to improve pipetting accuracy and efficiency. Methods The instrument pipetting target was set according to the sample volume of each reagent required specification. The differences between actual and target amount of sample were compared with quality pipette instrument,and continuously been amended. Finally a correction was set. Results We explored a STAR pipetting curve of ELISA reagents commonly used by blood station,set a correction for each common target value of ELISA detection of blood station,and optimize the pipetting sequence of 8 kinds of reagents tested and left template. Conclusion Using general parameters provided by factory or specific parameters set by engineer for users can cause some deviations between the target values the actual ones. To ensure accurate and efficient,users are required to set the correction and optimize the pipetting sequence according to the actual pipetting volume in the routine experimentation.

Key words: STAR pipetting instrument, System parameters setting, Optimization