Abstract: Objective To improvethe traditional frozen red blood cell technology，we have established a rapid，simple，high recovery rate of red blood cell freezing method. Methods The glycerol protective agent was divided into three parts，from low to high concentration and the total volume was constant. Three parts were added according to the sequence from low to high concentration. The recovery rate of red blood cells，free hemoglobin，and glycerol residue were compared between the traditional method and the improved method. Results Red blood cell recovery rate was （87.44 + 3.4）%，free hemoglobin was （0.65+0.31）g/L，glycerin residue was （4.2+0.75）g/L in improved method. The control group for the corresponding results was （81.77±3.2）%，（0.58±0.23）g/L and （2.5±0.34）g/L. The red cell recovery rate of the two .Methods was statistically different（P<0.05）. Conclusion The indexes of red blood cells prepared by the improved method were in line with the national standard. And the red blood cell recovery rate was higher than the traditional method. The quality of red blood cells was improved，which was vital for ensuring the safety of blood transfusion and improving the effect of blood transfusion.

Key words: Freezing-thawing red blood cell , Glycerol , Rate of recovery