Abstract: Objective To provide the reference basis for the development of regional scientific and effective recruiting blood donation and blood safety policy,blood test was carried out in unpaid blood donation population in foshan region during 2014-2016 . Methods Enzyme-linked immunoassay （enzyme immunoassay,EIA） joint nucleic acid detection technology （NAT） were used to detect donated blood collected from foshan area in 2014 to 2016without compensation in accordance with the provisions for testing,then the results were analyzed. Results The failure rate of total blood is decreasing year by year,and the difference in annual rate is statistically significant. Total percent of failure is 3.74%,unqualified rate of the conventional detection project from high to low in turn is ALT （1.97%）,HBsAg （0.91%）,anti - TP （0.35%）,anti-HCV （0.34%） and anti-HIV （0.18%）. The NAT failure rate was 0.25%,with decreased trend year by year,and the rate of the NAT reagent screening of 6 human specimens was significantly higher than that of 24 human specimens. ConclusionThe main factor causing unqualified unpaid blood donation population in foshan area is ALT and HBsAg,syphilis and HCV infected people have a certain proportion,the anti-HIV positive rate is increasing year by year,with increased risk of blood transmission of infectious diseases. Blood service organization should strengthen health examination and consultation before the blood donation in unpaid blood donation population,and choose more accurate,specific blood screening technology,improve the blood screening detection sensitivity,prevent blood transmission of infectious diseases to the greatest extent,ensure transfusion safety; At the same time blood stations should constantly improve the staff's service quality,strengthen propaganda and education of unpaid blood donors in donation knowledge,establish and develop low-risk,stable unpaid donors team,has the vital significance to blood safety.

Key words: Blood donors, EIA, NAT, Blood safety