Abstract: Objective To investigate blood donor samples of positive minipool-HBV NAT （MP-HBV）but negative resolution HBV NAT （resolution-HBV）whether contain trace HBV DNA. Methods Samples of positive MP-HBV but negative resolution-HBV,and positive resolution-HBV but negative quantitative HBV,and normal samples,were measured by electrochemiluminescence assay （ECLIA）for HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc. The resolution NAT was executed once more when resolution NAT was negative. Results Among 693 samples of positive MP-HBV with negative resolution-HBV,239 showed only anti-HBs,134 demonstrated anti-HBs/anti-HBc,111 with anti-HBs/anti-HBe/anti-HBc,25 with only anti-HBc and 9 with anti-HBe/anti-HBc. No significant difference of the seroprevalence was noted between these samples （74.75%）and normal samples （71.00%）. There were 119 of 275 resolution-HBV positive samples that presented quantitatively HBV negative. Among them,38 showed positive anti-HBe/anti-HBc,30 were anti-HBs/anti-HBc,22 anti-HBs/anti-HBe/anti-HBc,18 were only anti-HBc,3 only anti-HBs and 4 were HBsAg/ anti-HBe/anti-HBc. The HBV seroprevalence was 96.64%,which was higher than positive MP-HBV but negative resolution-HBV samples. Six of 29 secondary resolution NAT revealed positive HBV DNA. Conclusion There may exist HBV infection in positive MP-HBV but negative resolution-HBV samples with anti-HBs/anti-HBc.

Key words: Negative, Resolution NAT HBV, Serological markers