Analysis on Results of Different Reagent Kits of IgM and IgG in COVID-19 Convalescent Plasma

doi:10.3969/j.issn.1671-2587.2020.03.009

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2020, Vol. 22 ›› Issue (3): 258-263.DOI: 10.3969/j.issn.1671-2587.2020.03.009

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Analysis on Results of Different Reagent Kits of IgM and IgG in COVID-19 Convalescent Plasma

CHEN Fu-qiang, ZHU Hai-feng, XIAO Chen, et al

Blood Center of Shandong Province, Jinan 250014

Received:2020-03-23 Online:2020-06-20 Published:2020-06-13

Abstract

Abstract: Objective To compare ELISA and colloidal gold immunoassay reagents in IgG and IgM antibody titer detection in plasma from the COVID-19 convalescent patients (COVID-19 CP). To determine the effect of methylene blue/photochemical virus inactivation on the titer of 2019-nCoV IgG. Methods Blood samples were collected from 15 COVID-19 convalescent patients discharged from hospital in different time, and tested according to the blood station technical operation rules (2019 Edition). Novel coronavirus pneumonia (2019-nCoV) RNAs were detected by nucleic acid testing. 3 IgG and 2 IgM ELISA kits,1 colloidal gold reagent were used to detect the antibody of IgM and IgG respectively, after diluted by origin, 20, 40, 80, 160 and 320 times. The titers of IgG antibody were detected in 7 plasma samples before and after the virus inactivation by methylene blue to evaluate the effect of virus inactivation on the titer of antibody. Results 15 samples of COVID-19 CP were qualified, and the 2019-nCoV nucleic acid test was negative. The ALT levels were high. There was a significant difference in the titer of IgG detected by 3 IgG and 2 IgM ELISA reagent kits from different manufacturers. Conclusion Domestic reagents can detect the IgG and IgM of 2019-nCoV, but the titer levels are different from the different reagents. It is necessary to further study the methodology and sensitivity of qualitative reagent and gradient dilution method for the detection of antibody titer. Methylene blue/photochemical virus inactivation has no significant effect on the titer of 2019-nCoV IgG

Key words: ELISA, Colloidal gold immunochromatography, 2019-nCoV, IgG, IgM, Antibody titer, Methylene blue

CLC Number:

R446.1

CHEN Fu-qiang, ZHU Hai-feng, XIAO Chen, et al. Analysis on Results of Different Reagent Kits of IgM and IgG in COVID-19 Convalescent Plasma[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(3): 258-263.

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Analysis on Results of Different Reagent Kits of IgM and IgG in COVID-19 Convalescent Plasma

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