Abstract: Objective To research the effect of risk of blood transfusion in voluntary blood donors through investigating the results of the nucleic acid test of Hengyang．Methods From January 2015 to December 2016,84622 blood samples were collected from the donors and detected bytwo rounds of ELISA. Then,77610 blood samples,whether or not they were tested reactive by one reagent of ELISA,would be tested by NAT．The markers of HBV were detected in blood samples which had HBV-DNA . Results 217 blood samples out of 77610 were detected positive （at a rate of 0. 278%,216 blood samples were HBV-DNA positive,0 was HCV-RNA,1 was HIV-RNA．The NAT detection rate was less than that of ELISA （1.81％,1 533/84 622）. 86 HBV-DNA positive samples were detected by ELISA with HBcAb in 209 （41.15%） and significantly higher than the detection rate of HBcAb in the common population without HBSAg. Conclusion Resultssuggested that the implementation of NAT had effectively improved the blood safety and decreased the risk of blood transfusion of HBV in Hengyang. Blood banks shall consolidate and develop the low risk blood donors team to ensure the blood safety from source.

Key words: Nucleic acid test, ELISA, Risk of blood transfusion, Blood safety, COBASs201