Abstract: Objective The invalid results and listsmay influence thetimely sending ofthe nucleic acid test reports. This work aimed to explore the reason for the invalid results and to optimize the corresponding operations for safety of blood transfusion. Methods Blood samples were collected from the voluntarydonors in Anyang from Jun 2016 to may 2017and DNAs were extracted withHaoyuan ChiTas BSS1200 automatic nucleic acid separator and subsequently amplified with ABI7500 Fluorescent Amplifier. The invalid results and lists were analyzed. Results A total of 88739 samples were tested for DNAs and 0.06% results and 1.04% lists were found to be invalid. Further analyses of the invalid results showed that it is not the batche's number of reagent but the insufficient amounts of samples as well as personal operation errorsor laboratory contaminationsthat causedthe invalid amplification of internal standard. Conclusion The invalid results and lists can be minimized by enhancing the training of the staff who are involved in nucleic acid tests,improving operating procedures,and avoiding laboratory contaminations by daily cleaning and disinfecting.

Key words: Nucleic acid test, Invalid result, Invalid list