Abstract: Objective To research and analyze the serological characterization of occult hepatitis B virus infection in volunteer blood donors in Handan.Methods From March 1,2017 to December 31,2017,75,984 samples were screened by two ELISA reagents. The normal HBsAg single reagent positive specimens and negative specimens were used to undergo qualitative nucleic acid test. Then HBV DNA positive specimen were further determined by quatitative nucleic acid test and Chemical luminescence "double half" test,the correlation between combined two-method DNAtest resultsand blood donor statistics information was analysed.Results 26 OBI samples were obtained for HBsAg^-/ HBV DNA⁺ / HBcAb⁺,Among them,HBsAg^-/HBsAb⁺/HBcAb⁺ and HBsAg^-/HBsAb^-/HBcAb⁺ are the main serotypes of OBI,Each of these serotypes accounted for 30.8 % and 50.0 % respectively. The number of HBsAb>100IU/L samples accounted for 11.5 % of the total number of cases（3/26）,and the number of HBsAb （20-160） IU/L was 6 cases,accounting for 23.1 % of the total number of cases（6/26）. The concentration of the gradient dilution HBV DNA standard（3.15 IU/mL,4.2 IU/mL IU/mL,6.3 IU/mL,8.4 IU/mL） corresponds to the CT values of 41.14,37.45,36.23,and 35.04,respectively. There was no statistically significant difference between men and women in the rate of OBI infection. In terms of age distribution,there was an increasing trend with age.ConclusionAdoption of nucleic acid detection would reduce the risk of blood transfusion infection caused by the double ELISA method,especially for the detection of OBI,and improve the safety of blood transfusion. Encouragement of healthy blood donors with low anti-HBs titration to timely injection of hepatitis B vaccine would reduce the risk of blood transfusion infection of hepatitis B.

Key words: Occult hepatitis B virus, Nucleic acid test, Blood donors, Serological distribution