Abstract: Objective To analyze the results of nucleic acid detection in Weinan in 2016 and observe its significance in ensuring blood safety. Methods The Roche Cobas s201 automatic nucleic acid detection system was used to detect the specimens that had shown negative ELISA and qualified ALT. Results A total of 34251 cases were examined including 33 cases of positive HBV DNA. The HBV prevalence was found to be 0.96 ‰,HCV RNA and HIV RNA were not detectable. No significant difference of the positive rate of HBV DNA was noted between different months （χ²= 5.57,P>0.05）. The Ct value of positive rate in mixed detection was negatively correlated to positive rate on single test. There was significant difference between the groups with different Ct values （χ²= 14.50,P<0.01）. The HBV DNA prevalence varied in different age groups（χ²= 35.86,P<0.01）,among them the highest positive rate of HBV DNA was seen in 41~50 year old group （72.73% （24/33）. The HBV DNA-positive rates were statistically different between primary donors and repeated ones （χ²=9.94,P<0.01）. The HBV DNA prevalence in male donors was obviously higher than that in female donors （χ² = 6.08,P<0.05）. Conclusion Thirty three HBV DNA-positive cases were found in 34 251 samples with negative ELISA results in Weinan area. This suggesting the risk of blood-borne diseases at HBV in the voluntary blood donors. Nucleic acid detection in screening of blood donors may help to improve the safety of blood trasfusion.

Key words: Nucleic acid test, Window period, Blood transfusion safety