Abstract: Objective This study aimed at investigating the recalling of blood donors in Dalian who had been identified aspositiveanti-HCVcarriers by routine screening Methods.Methods Anti-HCV positive samples that had been preliminarily screened by routine screening were re-examined with ELISA,NAT and electro-chemiluminescence assay in Dalian Blood Center from January 2013 to July 2015. The time and intervalof recalling was evaluated. Results A total of 192 065 blood donorswere screened and 679（0.35%） cases wereprimarily identified to beanti-HCV positive. Among them77 cases were followed upby repeated combinative detections stated above. No significant difference of ELISA positive rate between various recalling times and intervals （P=0.106,P=0.353,respectively） was seen in the blood donors whoshowed both negative electro-chemiluminescence assay and NAT.Thus twotimes of recalling and longer than 3 monthsof interval weredeterminedas the appropriate recalling strategy. Conclusion The screen Methods routinely usedis insufficient to avoid the occurrence of false positivitiesthat might be related tounqualified regents and/or uncaliberated instruments. A deep approach is needed to optimize thestrategy of laboratory examinations for rejectionof the anti-HCV positive cases or rejoin of qualified blood donors in order toensure the safety of resource.

Key words: Rejoin of blood donors, Nucleic acid test, Anti-HCV