Abstract: Objective To analyze the confirmation test Results of anti-HCV screening-positive samples of the blood donors. Method The 284 anti-HCV screening-positive donor samples were collected randomly. Western blotting（WB） test was used to confirm the Results. The Results were evaluated based on the data of WB test and personal information of the blood donors. Result 49.82 percent of 284 cases of anti-HCV screening-positive donor samples were confirmed to be positvie. Compared with the confirmation Results of positive samples screened by two round ELISA and positive samples screened by single round ELISA ,significant statistical differences were noted in positive rates. The confirmed positive rates between initial and repeated blood donations,genders and ages revealed significant statistical differences. Conclusion Confirmation or additional tests should be carried out for screening-positive donors,particularly in those screened by single round ELISA and repeated blood donors.

Key words: Anti-HCV, Western Blot（WB）, ELISA