Abstract: Objective To Use the fluorescence quantitative PCR （FQ-PCR） for confirming antibodies against hepatitis C surface antigen（anti-HCV）that had been tested by enzyme linked immunoassay（ELISA）. Methods 1 348 patients who had experienced blood transfusion and operation were collected from April 2012 to March 2016.The patients were divided into three groups,group A included 240 samples with negative anti-HCV detected by ELISA （S/CO<0.3）; group B covered 902 samples with anti-HCV on cutoff value （0.7≤S/CO<1）; and group C included 206 samples with weak reaction of anti-HCV detected by ELISA. Results Among groups B and C,41（4.54%）and 172（83.49%） samples were found to have HCV RNA level>1 000 IU/ml rechecked by FQ-PCR. FQ-PCR demonstrated a high positive rate in the samples on the cutoff value tested by ELISA double reagents（P<0.05）; while no difference was noted between the samples with weak reaction of HCV RNA when tested with ELISA and FQ-PCR（P>0.05）. Conclusion The false negative and misdiagnosis rate of ELISA is high in the samples near HCV cutoff value and in weak reaction,suggesting that FQ-PCR is useful for early diagnose of HCV patients.

Key words: ELISA, Weak reactive, Anti-HCV, FQ-PCR